lncRNA CDKN2B-AS1靶向miR-98-5p对肺癌A549细胞增殖、侵袭的影响  

Effects of lncRNA CDKN2B-AS1 targeting miR-98-5p on proliferation and invasion of lung cancer A549 cells

作　　者：王思伟 曹秀芳 徐玉镇 姜波[1] 王晗[2] 文洁 Wang Siwei;Cao Xiufang;Xu Yuzhen;Jiang Bo;Wang Han;Wen Jie(Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Jining Medical College,Jining 272000,China;Department of Oncology,First People’s Hospital of Jining,Jining 272000,China;Jining Medical College,Jining 272113,China)

机构地区：[1]济宁医学院附属医院呼吸与危重症医学科,272000 [2]山东省济宁市第一人民医院肿瘤科,272000 [3]济宁医学院,272113

出　　处：《中华内分泌外科杂志》2021年第3期299-303,共5页Chinese Journal of Endocrine Surgery

基　　金：济宁医学院教师科研扶持基金(JYFC2018FKJ075)。

摘　　要：目的探讨长链非编码RNA(lncRNA)CDKN2B-AS1靶向miR-98-5p对肺癌A549细胞增殖、侵袭的影响。方法体外培养肺癌A549细胞,分为对照组、pcDNA组(转染pcDNA)、CDKN2B-AS1组(转染pcDNA CDKN2B-AS1)和双转染组(转染pcDNA CDKN2B-AS1、pcDNA miR-98-5p)。检测A549细胞中CD-KN2B-AS1、miR-98-5p表达及PCNA、MMP-9蛋白表达,检测A549细胞活性、克隆数、克隆形成率、侵袭细胞数。结果与pcDNA组相比,CDKN2B-AS1组A549细胞中CDKN2B-AS1表达水平[(2.14±0.14)vs(1.03±0.10)]、各时间点的细胞OD值、细胞克隆数[(314.60±18.13)个比(220.08±12.46)个]、克隆形成率[(85.81±3.06)%vs(60.03±2.85)%]、侵袭细胞数[(233.30±18.98)个vs(140.84±12.30)个]及细胞中PCNA[(0.78±0.08)vs(0.48±0.07)]、MMP-9蛋白表达[(0.75±0.06)vs(0.38±0.06)]水平显著升高(均P<0.05),miR-98-5p表达水平[(0.23±0.03)vs(0.99±0.09)]显著降低(P<0.05);与CDKN2B-AS1组相比,双转染组A549细胞中CD-KN2B-AS1表达水平差异无统计学意义(P>0.05),miR-98-5p表达水平显著升高(P<0.05),各时间点的细胞OD值、细胞克隆数、克隆形成率、侵袭细胞数及细胞中PCNA、MMP-9蛋白表达水平均显著降低(P<0.05)。结论lncRNA CDKN2B-AS1通过靶向抑制miR-98-5p表达对肺癌A549细胞增殖、侵袭具有促进作用。Objective To investigate the effects of long non-coding RNA(lncRNA)CDKN2B-AS1 targeting miR-98-5p on proliferation and invasion of lung cancer A549 cells.Methods A549 cells cultured in vitro were divided into control group,pcDNA group(transfected with pcDNA),CDKN2B-AS1 group(transfected with pcDNA CDKN2B-AS1)and double transfection group(transfected with pcDNA CDKN2B-AS1 and pcDNA miR-98-5p).The expression of lncRNA CDKN2B-AS1,miR-98-5p and the protein expression of PCNA,MMP-9 in A549 cells were detected.The activity,clone number,cloning efficiency,and the number of invasive cells of A549 cells were detected.Results Compared with pcDNA group,the expression level of lncRNA CDKN2B-AS1[(2.14±0.14)vs(1.03±0.10)],OD value in each time points,clone number[(314.60±18.13)vs(220.08±12.46)],cloning efficiency[(85.81±3.06)%vs(60.03±2.85)%],invasive cell number[(233.30±18.98)vs(140.84±12.30)],expression levels of PCNA[(0.78±0.08)vs(0.48±0.07)]and MMP-9[(0.75±0.06)vs(0.38±0.06)]proteins in A549 cells in CDKN2B-AS1 group were significantly increased(P<0.05);the expression level of miR-98-5p[(0.23±0.03)vs(0.99±0.09)]was significantly decreased(P<0.05);compared with CDKN2B-AS1 group,there was no significant difference in the expression level of lncRNA CDKN2B-AS1 in A549 cells in double transfection group(P>0.05),while the expression level of miR-98-5p in A549 cells was significantly increased(P<0.05).The OD value in each time points,clone number,cloning efficiency,invasive cell number,expression levels of PCNA and MMP-9 proteins were significantly decreased(P<0.05).Conclusion LncRNA CDKN2B-AS1 can promote the proliferation and invasion of lung cancer A549 cells by targetingly inhibiting the expression of miR-98-5p.

关 键 词：长链非编码RNA CDKN2B-AS1 miR-98-5p 肺癌A549细胞 增殖 侵袭 

分 类 号：R563[医药卫生—呼吸系统]