基于TiO_(2)纳米棒的表面增强拉曼技术检测细胞中端粒酶活性  

Surface-Enhanced Raman Scattering Technology Based on TiO_(2) Nanorods for Detection of Telomerase Activity in Cells

作　　者：刘晓燕 周琰 郑婷婷 田阳 LIU Xiao-Yan;ZHOU Yan;ZHENG Ting-Ting;TIAN Yang(School of Chemistry and Molecular Engineering,East China Normal University,Shanghai 200241,China;State Key Laboratory of Precision Spectroscopy,East China Normal University,Shanghai 200241,China)

机构地区：[1]华东师范大学化学与分子工程学院,上海200241 [2]华东师范大学,精密光谱科学与技术国家重点实验室,上海200241

出　　处：《分析化学》2021年第7期1218-1227,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21827814,21974049);上海市青年科技英才启明星计划项目(No.20QA1403300)资助。

摘　　要：利用水热法制备了TiO_(2)纳米棒(TiO_(2) nanorods,TiO_(2) NRs),构建了一种TiO_(2) NRs非金属表面增强拉曼光谱(Surface-enhanced Raman spectroscopy,SERS)生物传感器,以酞菁铜(Copper(Ⅱ)phthalocyanine,CuPc)为吸附分子,开发的TiO_(2) NRs SERS生物传感器具有显著的拉曼活性。通过实验数据和理论计算,发现CuPc分子显著的SERS增强(增强因子(Enhancement factor,EF)=3.18×10^(8))源于电荷转移的化学增强(Chemical mechanism,CM)机理。利用TiO_(2) NRs纳米阵列上CuPc的显著拉曼响应以及对端粒G-四连体的特异性识别,TiO_(2) NRs作为SERS基底,成功实现了对端粒酶活性的灵敏的定量检测,检测限低至2.85×10^(-16) IU/L。利用此SERS传感器的高选择性和高灵敏度等分析性能,测了定Hela细胞中端粒酶活性并确定其细胞数量,表明本方法为检测Hela等细胞中端粒酶活性的有效方式。本研究不仅为基于CM的半导体SERS增强机理研究奠定了基础,也为临床诊断中的相关物质检测和细胞生物医学分析开辟了新途径。The TiO_(2) nanorods(TiO_(2) NRs)were prepared by hydrothermal method,and a TiO_(2) NRs non-metallic surface-enhanced Raman spectroscopy(SERS)biosensor was constructed.With copper phthalocyanine(CuPc)as the adsorbed molecule,the developed TiO_(2) NRs SERS biosensor revealed remarkable Raman activity.Through experimental data and theoretical calculations,it was found that significant SERS enhancement(Enhancement factor(EF)=3.18×10^(8))of CuPc was due to the chemical mechanism(CM)based on charge transfer.By utilizing the significant Raman response of CuPc on the TiO_(2) NRs and the specific recognition of telomere G-quadruplex,TiO_(2) NRs was used as a SERS biosensor for quantitative and sensitive detection of telomerase activity,with a detection limit down to 2.85×10^(-16) IU/L.In addition,due to the high selectivity and high sensitivity,the SERS biosensor was used to determine the telomerase activity as well as the cell numbers in Hela cells,making it an effective way to detect telomerase activity in other cells.This work not only established an approach for studying the Raman enhancement mechanism of semiconductor based on CM,but also paved a new way for the detection of related substances in clinical diagnosis and cell biomedical analysis.

关 键 词：表面增强拉曼光谱 TiO_(2)纳米棒 电荷转移增强 端粒酶活性 HELA细胞 

分 类 号：R318[医药卫生—生物医学工程] O657.37[医药卫生—基础医学] TB383.1[理学—分析化学]