Lightweight 3D bioprinting with point by point photocuring  

作　　者：Peng Zhang Haoxuan Wang Peng Wang Yating Zheng Linxiang Liu Jun Hu Yande Liu Qing Gao Yong He 

机构地区：[1]School of Mechatronics&Vehicle Engineering,East China Jiaotong University,Nanchang,330013,China [2]State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou 310027,China [3]Engineering for Life Group(EFL),Suzhou,215000,China [4]Zhejiang University Hospital,Zhejiang University,Hangzhou,Zhejiang 310027,China

出　　处：《Bioactive Materials》2021年第5期1402-1412,共11页生物活性材料（英文）

基　　金：support of National 863 Program(SS2012AA101306);“the 12th Five-Year Plan”,Jiangxi Advantageous Science and Technology Innovation Team Construction Plan(20153BCB24002);Collaborative Innovation Center Project of Intelligent Management Technology and Equipment for Southern Mountain Orchards(G.J.G.Z.[2014]No.60);National Natural Science Foundation of China(2002017018,51805474);the China Postdoctoral Science Foundation(Grant No.2019T120509).

摘　　要：As photocrosslinkable materials,methacryloyl-modified hydrogels are widely used as bioinks in tissue engineering.Existing printing methods to use these hydrogels,including changing the viscosity of the material or mixing them with other printing components,have been explored,but their application has been limited due to low printing quality or high cost.In addition,the complex operation of bulky equipment restricts the application of these existing printing methods.This study presents a lightweight stereolithography-based three-dimensional(3D)bioprinting system with a smart mechanical and structural design.The developed bioprinter dimensions were 300 mm×300 mm×200 mm and it can be placed on a benchtop.The equipment has a mini bioink chamber to store a small amount of bioink for each printing.We systematically investigated the point-by-point curing process in the 3D bioprinting method,which can print mixed cells accurately and have good biocompatibility.Here,we provide a compact,low-cost stereolithography bioprinting system with excellent biocompatibility for 3D bioprinting with methacryloyl-modified hydrogels.It can be potentially used for drug screening,studying pathological mechanisms,and constructing biological disease models.

关 键 词：Three-dimensional(3D)bioprinting Stereolithography bioprinting system Compact Low-cost Tissue engineering Cell printing 

分 类 号：R318[医药卫生—生物医学工程]