CCL5、CXCL10和CXCL13在重度脑外伤患者中的表达和意义  

Expression and significance of chemokines in patients with severe traumatic brain injury

作　　者：刘苏[1] 陆纯 黄欢 孙丽[1] 王司晔[1] LIU Su;LU Chun;HUANG Huan;SUN Li;WANG Siye(Department of Rehabilitation,the Affiliated Hospital of Nantong University,Nantong 226001;School of Medicine,Nantong University)

机构地区：[1]南通大学附属医院康复医学科,南通226001 [2]南通大学医学院

出　　处：《南通大学学报（医学版）》2021年第2期119-122,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金青年基金资助项目(81702223);南通市科技计划项目(MS22019006)。

摘　　要：目的:观察趋化因子配体5(chemokine C-C motif ligand 5,CCL5)、趋化因子CXC配体[chemokine(C-X-C motif)ligand,CXCL]10、CXCL13在创伤性脑损伤(traumatic brain injury,TBI)患者外周血液中的表达变化,分析这些趋化因子对损伤程度和预后的影响。方法:选取39例重度TBI患者[格拉斯哥昏迷评分(Glasgow coma scale,GCS)3~8分]与13例健康对照者。酶联免疫吸附测定法检测TBI患者术后1、3、7 d和对照组外周血中CCL5、CXCL10、CXCL13蛋白含量;Spearman相关分析对不同时间点外周血中CCL5、CXCL10、CXCL13和入院GCS、术后30 d GCS和格拉斯哥结局量表评分(Glasgow outcome scale,GOS)进行相关性分析。结果:与对照组相比,重度TBI患者外周血液中CCL5、CXCL10、CXCL13的表达量均明显升高(均P<0.05)。重度TBI患者术后1 d血液中CCL5、CXCL13的蛋白浓度与入院GCS评分呈负相关(P<0.05),术后3 d CXCL10的浓度与术后30 d GCS评分呈负相关(P<0.05)。重度TBI患者术后1 d CCL5的蛋白含量、术后1 d和3 d CXCL10蛋白含量及术后7 d CXCL13的蛋白含量与术后30 d GOS评分呈负相关(P<0.05)。结论:趋化因子CCL5、CXCL10、CXCL13在TBI早期表达量迅速增加,且表达量越高,损伤程度越重,预后可能越差。Objective:To detect the levels of chemokines chemokine C-C motif ligand 5(CCL5),chemokine(C-X-C motif)ligand(CXCL)10 and CXCL13 in the peripheral blood of TBI patients and to analyze the effects of these chemokines on the degree of injury and prognosis.Methods:Thirty-nine patients with TBI[Glasgow coma scale(GCS)score 3-8]and 13 healthy people were selected.Enzyme linked immunosorbent assay(ELISA)method was used to detect the protein content of CCL5,CXCL10,and CXCL13 in peripheral blood of TBI patients at 1,3,and 7 days after surgery and the control group.Spearman correlation analysis was used to analyze the correlation among CCL5,CXCL10,CXCL13 in peripheral blood at different time points with admission GCS,GCS at 30 days after surgery and Glasgow outcome scale(GOS)scores at 30 days after surgery.Results:Compared with the control group,the levels of CCL5,CXCL10 and CXCL13 in the peripheral blood of patients with severe TBI were significantly increased(P<0.05).The protein concentration of CCL5 and CXCL13 in the blood of patients with severe TBI one day after surgery was negatively correlated with the admission GCS score(P<0.05),and the concentration of CXCL10 on the 3rd day after surgery was negatively correlated with the GCS score on the 30th day after surgery(P<0.05).In patients with severe TBI,the protein content of CCL5 at 1 day after surgery,the protein content of CXCL10 at 1 and 3 days after surgery,and the protein content of CXCL13 at 7 days after surgery were negatively correlated with the GOS score at 30 days after surgery(P<0.05).Conclusion:The levels of chemokines CCL5,CXCL10,and CXCL13 increased rapidly in the early stage of TBI,and the higher levels of CCL5,CXCL10,and CXCL13,the more severe injury and the worse the prognosis.

关 键 词：创伤性脑损伤 趋化因子配体5 趋化因子CXC配体10 趋化因子CXC配体13 神经炎症 

分 类 号：R651.15[医药卫生—外科学]