PTEN在颅底脊索瘤患者中的表达及其与临床病理学和预后的关系  

Correlation of PTEN Expression with Clinicopathology and Prognosis in Skull Base Chordoma

作　　者：田凯兵[1] 王亮[1] 马骏鹏 王科[1] 张俊廷[1] 吴震[1] TIAN Kaibing;WANG Liang;MA Junpeng;WANG Ke;ZHANG Junting;WU Zhen(Department of Neurosurgery,Beijing Tiantan Hospital Affiliated to Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学附属北京天坛医院神经外科,北京100070

出　　处：《医学综述》2021年第12期2462-2467,共6页Medical Recapitulate

基　　金：国家自然科学基金(81802683)。

摘　　要：目的探讨人第10号染色体缺失的磷酸酶及张力蛋白同源物(PTEN)在颅底脊索瘤中的表达及其与临床病理学和预后的关系。方法选择2008年1月至2015年11月在首都医科大学附属北京天坛医院神经外科接受手术治疗的颅底脊索瘤患者53例,分别应用石蜡样本切片进行免疫组织化学染色、冰冻组织样本进行实时定量聚合酶链反应检测PTEN蛋白和基因的表达,分析PTEN表达与颅底脊索瘤临床病理学和预后的关系。结果PTEN蛋白低表达者26例,高表达者27例。PTEN蛋白表达在颅底脊索瘤患者不同性别、分期、分隔、切除方式及病理类型方面差异无统计学意义(P>0.05);非骨质侵袭肿瘤中PTEN蛋白高表达率低于骨质侵袭肿瘤[22.22%(6/27)比77.78%(21/27)],质地软的肿瘤中PTEN蛋白高表达率高于质地硬的肿瘤[55.56%(15/27)比44.44%(12/27)](P<0.05)。Kaplan-Meier分析结果显示,PTEN蛋白低表达者术后无进展生存期(PFS)较高表达者显著缩短[(9.00±1.91)个月比(35.00±3.18)个月](P<0.05)。PTEN基因表达与颅底脊索瘤患者临床病理学无关联。结论在颅底脊索瘤中,非骨侵袭肿瘤PTEN蛋白高表达率低,质地软的肿瘤PTEN蛋白高表达率高,PTEN蛋白低表达者PFS缩短。Objective To investigate the expression of phosphatase and tensin homolog deleted on chromosome ten(PTEN)in skull base chordoma and its correlation with the clinicopathology and prognosis.Methods A total of 53 patients with skull base chordoma receiving surgery in Department of Neurosurgery of Beijing Tiantan Hospital Affiliated to Capital Medical University from Jan.2008 to Nov.2015 were enrolled.Immunohistochemical staining for paraffin samples and real-time quantitative polymerase chain reaction for frozen samples were used to detect the protein and gene expression of PTEN,and the correlation of the PTEN expression with the clinicopathological features and prognosis was analyzed.Results At the protein level,PTEN protein expression was low in 26 cases and high in 27 cases.There was no significant difference in the expression of PTEN protein between the patients with different genders,stages,separation,resection methods and pathological types(P>0.05).The rate of high PTEN level in the non-bone invasive tumors was lower than that in the bone invasive tumors[22.22%(6/27)vs 77.78%(21/27)];the rate of high PTEN level in the soft tumors was higher than that in the hard tumors[55.56%(15/27)vs 44.44%(12/27)](P<0.05).Kaplan-Meier analysis showed that patients with low PTEN protein expression had significantly shorter progression-free survival(PFS)than those with high PTEN protein expression[(9.00±1.91)months vs(35.00±3.18)months]P<0.05).At the gene level,PTEN expression was not correlated with the clinicopathology.Conclusion In skull base chordoma,the rate of high PTEN protein expression in the non-bone invasive tumors is lower,in the soft tumor is higher,and PFS of the patients with low PTEN protein expression is significantly shortened.

关 键 词：颅底 脊索瘤 人第10号染色体缺失的磷酸酶及张力蛋白同源物 

分 类 号：R651.1[医药卫生—外科学]