Surface modification of electrospun fibers with mechano-growth factor for mitigating the foreign-body reaction  

作　　者：Yang Song Linhao Li Weikang Zhao Yuna Qian Lili Dong Yunnan Fang Li Yang Yubo Fan 

机构地区：[1]Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing,100083,PR China [2]Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University,Chongqing,400030,PR China [3]Department of Bioengineering,University of California,Los Angeles,CA,90095,USA [4]Department of Orthopedics,The First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,PR China [5]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,325001,PR China

出　　处：《Bioactive Materials》2021年第9期2983-2998,共16页生物活性材料（英文）

基　　金：We acknowledge the funding support from the National Natural Science Foundation of China(31971258,11532004);the 111 Project(B06023,B13003);Chongqing Postdoctoral Science Foundation(csts2019jcyj-bsh0068).

摘　　要：The implantation of synthetic polymeric scaffolds induced foreign-body reaction(FBR)seriously influence the wound healing and impair functionality recovery.A novel short peptide,mechano-growth factor(MGF),was introduced in this study to modify an electrospun polycaprolactone(PCL)fibrous scaffold to direct the macrophage phenotype transition and mitigate the FBR.In vitro studies discovered the cell signal transduction mechanism of MGF regulates the macrophage polarization via the expression of related genes and proteins.We found that macrophages response the MGF stimuli via endocytosis,then MGF promotes the histone acetylation and upregulates the STAT6 expression to direct an anti-inflammatory phenotype transition.Subsequently,an immunoregulatory electrospun PCL fibrous scaffold was modified by silk fibroin(SF)single-component layer-by-layer assembly,and the SF was decorated with MGF via click chemistry.Macrophages seeded on scaffold to identify the function of MGF modified scaffold in directing macrophage polarization in vitro.Parallelly,rat subcutaneous implantation model and rat tendon adhesion model were performed to detect the immunomodulatory ability of the MGF-modified scaffold in vivo.The results demonstrate that MGF-modified scaffold is beneficial to the transformation of macrophages to M2 phenotype in vitro.More importantly,MGF-functionalized scaffold can inhibit the FBR at the subcutaneous tissue and prevent tissue adhesion.

关 键 词：Mechano-growth factor(MGF) SILK Macrophage polarization Foreign-body reactions(FBR) ELECTROSPINNING 

分 类 号：R318[医药卫生—生物医学工程]