蝴蝶兰‘红箭’组织培养快繁技术  

Tissue Culture and Rapid Propagation of A New Phalaenopsis Cultivar′Red Arrow′

作　　者：陈春[1] CHEN Chun(Fujian Forestry Science and Technology Test Center,Nanjing 363600,Fujian,China)

机构地区：[1]福建省林业科技试验中心,福建南靖363600

出　　处：《福建林业科技》2021年第2期63-67,共5页Journal of Fujian Forestry Science and Technology

基　　金：福建省2020年中央财政林业科技推广示范项目(闽[2020]TG14号)。

摘　　要：以花梗为外植体,开展蝴蝶兰‘红箭’诱导、增殖、生根培养及组培苗移栽等研究。结果表明,花梗最佳处理为75%酒精消毒45 s后,0.1%升汞消毒25 min;最佳诱导培养基为MS+6-BA 5.0 mg·L^(-1)+Ad 5.0 mg·L^(-1)+NAA 0.6 mg·L^(-1)+蔗糖30 g·L^(-1),诱导率达83.5%;最佳增殖培养基为MS+6-BA 7.0 mg·L^(-1)+Ad 5.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+香蕉80 g·L^(-1)+土豆80 g·L^(-1)+蔗糖30 g·L^(-1),增殖系数达3.36;最佳生根诱导培养基为1/2 MS+NAA 0.5 mg·L^(-1)+香蕉80 g·L^(-1)+土豆80 g·L^(-1)+活性炭1.0 g·L^(-1)+蔗糖15 g·L^(-1),生根率达100%;水苔为基质,移栽成活率可达95.2%。With pedicel as explants,the induction,proliferation,rooting culture and tissue culture of Phalaenopsis′Red arrow′were studied.The results showed that the best treatment of pedicel was disinfection with 75%alcohol for 45s and 0.1%mercuric chloride for 25 min.The optimal induction-cultured group was MS+6-BA 5.0 mg·L^(-1)+Ad 5.0 mg·L^(-1)+NAA 0.6 mg·L^(-1)+sucrose 30 g·L^(-1),with the induction-inductivity reaching 83.5%.The optimal medium for proliferation was MS+6-BA 7.0 mg·L^(-1)+Ad 5.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+banana 80 g·L^(-1)+potato 80 g·L^(-1)+sucrose 30 g·L^(-1),with a proliferation coefficient of 3.36.The optimal rooting medium was 1/2 MS+NAA 0.5 mg·L^(-1)+banana 80 g·L^(-1)+potato 80 g·L^(-1)+C 1.0 g·L^(-1)+sucrose 15 g·L^(-1) with rooting rate up to 100%.Water moss as substrate,transplant survival rate can reach 95.2%.

关 键 词：红箭 组织培养 快繁 组培苗移栽 

分 类 号：S682.31[农业科学—观赏园艺] S723.132.6[农业科学—园艺学]