Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy  

作　　者：Yang Liu Zhongying Rui Wei Cheng Licheng Song Yunqiang Xu Ruixin Li Xizheng Zhang 

机构地区：[1]Department of Orthopedics,Tianjin Medical University General Hospital,Tianjin 300052,China [2]Institute of Medical Service Support,Institute of Systems Engineering,Academy of Military Sciences,Tianjin,China [3]Department of Nuclear Medicine,Tianjin Medical University General Hospital,Tianjin 300052,China [4]Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction,Tianjin Stomatological Hospital,The Affiliated Stomatological Hospital of Nankai University,Tianjin 300041,China

出　　处：《Regenerative Biomaterials》2021年第2期104-117,共14页再生生物材料（英文版）

基　　金：The authors acknowledge financial support from the National Natural Science Foundation of China(11972198,31470935 and 11432016).

摘　　要：Cell–material interactions during early osseointegration of the bone–implant interface are critical and involve crosstalk between osteoblasts and osteoclasts.The surface properties of titanium implants also play a critical role in cell–material interactions.In this study,femtosecond laser treatment and sandblasting were used to alter the surface morphology,roughness and wettability of a titanium alloy.Osteoblasts and osteoclasts were then cultured on the resulting titanium alloy disks.Four disk groups were tested:a polished titanium alloy(pTi)control;a hydrophilic micro-dislocation titanium alloy(sandblasted Ti(STi));a hydrophobic nano-mastoid Ti alloy(femtosecond laser-treated Ti(FTi));and a hydrophilic hierarchical hybrid micro-/nanostructured Ti alloy[femtosecond laser-treated and sandblasted Ti(FSTi)].The titanium surface treated by the femtosecond laser and sandblasting showed higher biomineralization activity and lower cytotoxicity in simulated body fluid and lactate dehydrogenase assays.Compared to the control surface,the multifunctional titanium surface induced a better cellular response in terms of proliferation,differentiation,mineralization and collagen secretion.Further investigation of macrophage polarization revealed that increased anti-inflammatory factor secretion and decreased proinflammatory factor secretion occurred in the early response of macrophages.Based on the above results,the synergistic effect of the surface properties produced an excellent cellular response at the bone–implant interface,which was mainly reflected by the promotion of early ossteointegration andmacrophage polarization.

关 键 词：femtosecond laser hybrid micro-/nanostructure cell–material interactions OSSEOINTEGRATION macrophage polarization 

分 类 号：TG146.23[一般工业技术—材料科学与工程]