Sodium alendronate loaded poly(L-lactideco-glycolide)microparticles immobilized on ceramic scaffolds for local treatment of bone defects  

作　　者：Łucja Rumian Cornelia Wolf-Brandstetter Sina Ro¨ßler Katarzyna Reczynska Hanna Tiainen Ha˚vard JHaugen Dieter Scharnweber Elzbieta Pamuła 

机构地区：[1]Faculty of Materials Science and Ceramics,Department of Biomaterials and Composites,AGH University of Science and Technology,Al.A.Mickiewicza 30,Krakow 30-059,Poland [2]Technische Universita¨t Dresden,Institute of Materials Science,Max Bergmann Center of Biomaterials,Budapester Str.27,Dresden 01-069,Germany [3]Department of Biomaterials,Institute for Clinical Dentistry,University of Oslo,Geitmyrsveien 71,Blindern,P.O.Box 1109,Oslo NO-0317,Norway

出　　处：《Regenerative Biomaterials》2021年第3期22-31,共10页再生生物材料（英文版）

基　　金：This work was supported by National Science Centre,Poland(2013/09/N/ST8/00309);Norwegian Research Council(228415);BMBF,Germany(GoBone German-Polish bilateral project 01DS16010A)。

摘　　要：Bone tissue regeneration in critical-size defects is possible after implantation of a 3D scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing.Sodium alendronate(Aln),a widely used anti-osteoporosis drug,exhibits strong inhibitory effect on bone resorption performed by osteoclasts.Thus,we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide scaffolds and poly(L-lactide-co-glycolide)microparticles(MPs)loaded with Aln.The MPs were effectively attached to the surface of the scaffolds’pore walls by human recombinant collagen.Drug release from the scaffolds was characterized by initial burst(2466%of the drug released within first 24 h)followed by a sustained release phase(on average 5 mg of Aln released per day from Day 3 to Day 18).In vitro tests evidenced that Aln at concentrations of 5 and 2.5 mg/ml was not cytotoxic for MG-63 osteoblast-like cells(viability between 8166%and 9863%of control),but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells,as shown by reduced fusion capability and decreased tartrateresistant acid phosphatase 5b activity(5665%reduction in comparison to control after 8 days of culture).Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis,reducing osteoclast activity,but not affecting osteoblast functions,which may be beneficial in the treatment of critical-size bone tissue defects.

关 键 词：ceramic scaffolds sodium alendronate osteoblasts osteoclastogenesis collagen critical-size defect poly(L-lactideco-glycolide) MICROPARTICLES 

分 类 号：R318[医药卫生—生物医学工程]