pH敏感的PLGA包覆Ag_(2)S团簇用于近红外Ⅱ区荧光引导的肝癌手术切除  

pH sensitive Ag_(2)S loaded PLGA nanoclusters for NIR-Ⅱ imaging guided surgical removal of hepatocellular carcinoma

作　　者：靳玉慎 唐初 邵兵 JIN Yushen;TANG Chu;SHAO Bing(Central Research Laboratory,Beijing Centre for Disease Preventive Medical Research,Beijing 100013,China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control,Beijing 100013,Beijing,China;Engineering Research Center of Molecular and Neuro Imaging,Ministry of Education,School of Life Science and Technology,Xidian University,Xi'an 710126,Shaanxi,China)

机构地区：[1]北京市预防医学研究中心中心实验室,北京100013 [2]北京市疾病预防控制中心,食物中毒诊断溯源技术北京市重点实验室,北京100013 [3]西安电子科技大学生命科学技术学院,教育部分子与神经成像工程研究中心,陕西西安710126

出　　处：《精细化工》2021年第6期1155-1161,共7页Fine Chemicals

基　　金：国家自然科学基金面上项目(82072098);国家自然科学基金(81701771)。

摘　　要：通过共沉淀法和化学键偶联技术制备出肝癌靶向的pH敏感的装载Ag_(2)S的聚乳酸-羟基乙酸(SP94-PLGA@Ag_(2)S)纳米粒子,将其用于近红外(NIR)Ⅱ区荧光引导的肝癌手术切除。体内外实验结果表明,在酸性条件下(pH 6.5),SP94-PLGA@Ag_(2)S纳米粒子会发生降解,导致Ag_(2)S纳米粒子间距增大,荧光恢复(荧光强度百分比从20%恢复到100%),能够实现肝癌组织与正常组织的精准区分。此外,注射SP94-PLGA@Ag_(2)S纳米粒子后,在近红外Ⅱ区荧光手术导航设备的辅助下,可实现肝癌的有效切除,大大延长了荷瘤小鼠的生存周期(术后60 d生存率由传统手术组的40%提高到了90%),可有效降低术后复发率。Hepatocellular carcinoma targeted and pH-sensitive Ag_(2)S loaded poly lactic-co-glycolic acid SP94-PLGA@Ag_(2)S nanoparticles were prepared by coprecipitation and chemical bond combination method for near-infrared region Ⅱ fluorescence-guided surgical resection of hepatocellular carcinoma. The in vitro and in vivo experimental results showed that SP94-PLGA@Ag_(2)S nanoparticles tended to produce degradation under acidic condition(pH 6.5), leading to the increase of the distance between Ag_(2)S nanoparticles and the recovery of fluorescence(the percentage of fluorescence intensity was restored from 20% to 100%). The degradation of SP94-PLGA@Ag_(2)S nanoparticles could realize the accurate differentiation between hepatocellular carcinoma tissues and normal tissues. In addition, the injection of SP94-PLGA@Ag_(2)S nanoparticles with the assistance of near-infrared region(NIR) Ⅱ fluorescent surgical navigation equipment could effectively remove hepatocellular carcinoma, which greatly prolonged the lifetime(compared with that of the traditional surgery, the survival rate at 60 days after surgery increased from 40% to 90%) and effectively reduced the postoperative recurrence rate.

关 键 词：PH敏感 Ag_(2)S纳米粒子 近红外Ⅱ区荧光成像 肝癌 手术切除 功能材料 生物工程 

分 类 号：R318.08[医药卫生—生物医学工程] R735.7[医药卫生—基础医学]