医院感染嗜麦芽窄食单胞菌的分子分型研究  

Molecular Typing of Stenotrophomonas Maltophilia in a Hospital

作　　者：宋惠珠 李青青 潘瑞琳 徐玉妹 耿先龙 SONG Hui-zhu;LI Qing-qing;PAN Rui-lin;XU Yu-mei;GENG Xian-long(Department of Pharmacy,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi,Jiangsu,214023,China;Department of Clinical Laboratory,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi,Jiangsu,214023,China)

机构地区：[1]南京医科大学附属无锡人民医院药剂科,江苏无锡214023 [2]南京医科大学附属无锡人民医院医学检验科,江苏无锡214023

出　　处：《现代生物医学进展》2021年第11期2125-2128,共4页Progress in Modern Biomedicine

基　　金：无锡市医院管理中心科技发展基金项目(YGM1001)。

摘　　要：目的:对医院感染分离的嗜麦芽窄食单胞菌进行耐药谱和分子分型研究分析,为制订合理的感染控制方案提供依据。方法:对临床分离的42株嗜麦芽窄食单胞菌用质谱仪进行鉴定并分析其耐药表型,根据其对头孢他啶、复方新诺明、左氧氟沙星、米诺环素及头孢吡肟5种抗生素的药敏结果的不同分组,药敏结果相同的菌株分为一型,药敏结果不同的分为另一型;采用PFGE对其做分子分型分析。结果:42株嗜麦芽窄食单胞菌分为6个不同的耐药表型;对42个株菌株做PFGE电泳,共得到A-D 4个基因型。结论:嗜麦芽窄食单胞菌耐药谱特征和PFGE分型检测结果对抗生素选择性治疗有很好的指导作用。Objective: To study and analyze the drug resistance spectrum and molecular typing of Stenotrophomonas maltophilia in hospital infection, so as to provide basis for making reasonable infection control plan. Methods: 42 strains of clinical isolates of Stenotrophomonas maltophilia were identified by mass spectrometer and their drug resistance phenotypes were analyzed. According to the different groups of drug sensitivity results of five antibiotics, ceftazidime, compound neoformamine, levofloxacin, minocycline and cefepime, the strains with the same drug sensitivity results were divided into one type and the strains with different drug sensitivity results were divided into another type;PFGE was used for molecular typing Analysis. Results: 42 strains of Stenotrophomonas maltophilia were divided into 6 types. 42 strains of Stenotrophomonas maltophilia were analyzed by PFGE and 4 genotypes of A-D were obtained.Conclusions: The characteristics of drug resistance spectrum and PFGE typing of Stenotrophomonas maltophilia are very useful for the selective treatment of antibiotics.

关 键 词：嗜麦芽窄食单胞菌 感染 脉冲场凝胶电泳 

分 类 号：R927.1[医药卫生—药学] R197.323.4