油棕多胺合成酶基因的分离及其在低温和脱水胁迫下的表达分析  

Identification and Expressions under Low-temp/Draught Stress of Polyamine Synthesis Genes in Oil Palms

作　　者：金龙飞 尹欣幸 张安妮 曹红星[1] JIN Longfei;YIN Xinxing;ZHANG Anni;CAO Hongxing(Coconut Research Institute,Chinese Academy of Tropical Agricultural Science/Hainan Key Laboratory of Tropical Oil Crops Biology,Wenchang,Hainan 571339,China)

机构地区：[1]中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌571339

出　　处：《福建农业学报》2021年第4期402-411,共10页Fujian Journal of Agricultural Sciences

基　　金：中国热带农业科学院基本科研业务费专项(1630152017008)。

摘　　要：【目的】研究多胺合成酶基因在油棕生长发育及非生物胁迫应答中的作用,为油棕抗性品种培育提供理论基础。【方法】从油棕基因组中鉴定出15个编码多胺合成酶的基因,并对其进行生物信息学分析和表达模式分析。【结果】多胺合成酶的氨基酸长度为318~720,分子量为36.00~46.43 kDa,等电点为4.78~6.31,蛋白不稳定指数为29.26~48.73,脂溶指数为76.46~94.41,总平均亲水性为-0.315~0.082,外显子数目为1~11个,具有特定的蛋白保守基序和保守结构域。进化分析发现油棕多胺合成酶基因可以分为3个家族,EgADCs与水稻的亲缘关系较近,EgACLs、EgSPDSs与椰枣的亲缘关系较近,EgSPMSs与玉米的亲缘关系较近。启动子上鉴定出大量植物激素响应、逆境胁迫响应和光响应的顺式作用元件。组织表达分析发现多胺合成酶基因在油棕不同组织中均有表达;多胺合成酶基因受低温和脱水胁迫的诱导。【结论】多胺合成酶基因参与油棕对低温和脱水胁迫的应答,其中EgSPDS1、EgSPDS2、EgSPMS1、EgADC1、EgADC2、EgACL5-2、EgACL5-3、EgSAMDC3-1、EgSAMDC4-1、EgSAMDC4-2和EgACL5-1受低温胁迫的诱导,EgSPDS1、EgSPDS2、EgADC1、EgADC2、EgSAMDC4-1和Eg SAMDC4-2、EgACL5-1受脱水胁迫的诱导。【Objective】Genetic information and resource of low-temp/draught resistant oil palms were studied with respect to the stress responses of polyamines synthase gene in the plants for breeding purposes.【Method】Bioinformatics of polyamines synthase genes in oil palm were gathered from the genome bank,and their expressions under abiotic stresses analyzed.【Result】Fifteen polyamines synthase genes were identified from the oil palm genome.There were between 318 and 720 amino acids in the genes with a molecular weight ranging from 36.00 kDa to 46.43 kDa and an isoelectric point between 4.78 and 6.31.The instability indices of the genes were found in between 29.26 and 48.73,the aliphatic indices in between 76.46 and 94.41,the gravy in between-0.315 and 0.082,and the exon number in between 1 and 11.They contained both specific conserved protein motifs and conserved domain.A phylogenetic analysis divided them into 3 families,i.e.,EgADCs closely related to rice,EgACLs and EgSPDSs closely related to data palm,and EgSPMSs closely related to maize.Many cis-acting elements that response to phytohormone,stresses,and light were found in the promoters.The expressions of the polyamine synthase genes existed in different tissues of the plant and could be induced by either low temperature or draught stress.Specifically,EgSPDS1,EgSPDS2,EgSPMS1,EgADC1,EgADC2,EgACL5-2,EgACL5-3,EgSAMDC3-1,EgSAMDC4-1,EgSAMDC4-2,and EgACL5-1 were found to be upregulated by the imposed cold stress;while EgSPDS1,EgSPDS2,EgADC1,EgADC2,EgSAMDC4-1 EgSAMDC4-2,and EgACL5-1 by dehydration.【Conclusion】The polyamine synthase genes were significantly expressed in tissues of the oil palms that could be induced by either low temperature or draught stress.Those responded to the abiotic stresses were identified in this study.

关 键 词：油棕 多胺合成酶 低温 脱水 基因表达 

分 类 号：S718.43[农业科学—林学]