Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules  

作　　者：Na Li Wei Cui Peifang Cong Jie Tang Yong Guan Caihao Huang Yunen Liu Chengzhong Yu Rui Yang Xing Zhang 

机构地区：[1]Institute of Metal Research,Chinese Academy of Sciences,Shenyang,Liaoning,110016,China [2]School of Materials Science and Engineering,University of Science and Technology of China,Hefei,Anhui,230026,China [3]Department of Emergency Medicine,General Hospital of Northern Theater Command,Shenyang,Liaoning,110016,China [4]National Synchrotron Radiation Laboratory,University of Science and Technology of China,Hefei,Anhui,230029,China [5]Australian Institute for Bioengineering and Nanotechnology,The University of Queensland,Brisbane,QLD,4072,Australia

出　　处：《Bioactive Materials》2021年第8期2303-2314,共12页生物活性材料（英文）

基　　金：supported by the National Key Research and Development Program of China from Ministry of Science and Technology(2016YFC1100502);Key Research Program of Frontier Sciences(QYZDY-SSW-JSC031);from Chinese Academy of Sciences(CAS),Key Research and Development Program of Liaoning Province(201703031);Shenyang Key R&D and Technology Transfer Program(Z17-7-023).

摘　　要：Amorphous calcium phosphate(ACP)has been widely found during bone and tooth biomineralization,but the meta-stability and labile nature limit further biomedical applications.The present study found that the chelation of polyacrylic acid(PAA)molecules with Ca^(2+)ions in Mg-ACP clusters(~2.1±0.5 nm)using a biomineralization strategy produced inorganic-organic Mg-ACP/PAA hybrid nanoparticles with better thermal stability.Mg-ACP/PAA hybrid nanoparticles(~24.0±4.8 nm)were pH-responsive and could be efficiently digested under weak acidic conditions(pH 5.0-5.5).The internalization of assembled Mg-ACP/PAA nanoparticles by MC3T3-E1 cells occurred through endocytosis,indicated by laser scanning confocal microscopy and cryo-soft X-ray tomography.Our results showed that cellular lipid membranes remained intact without pore formation after Mg-ACP/PAA particle penetration.The assembled Mg-ACP/PAA particles could be digested in cell lysosomes within 24 h under weak acidic conditions,thereby indicating the potential to efficiently deliver encapsulated functional molecules.Both the in vitro and in vivo results preliminarily demonstrated good biosafety of the inorganic-organic Mg-ACP/PAA hybrid nanoparticles,which may have potential for biomedical applications.

关 键 词：Inorganic-organic hybrid pH responsive ACP clusters Cellular degradation Cryo-soft X-ray tomography 

分 类 号：TB383[一般工业技术—材料科学与工程]