Bioproduced Proteins On Demand(Bio-POD)in hydrogels using Pichia pastoris  

作　　者：Shuo-Fu Yuan Sierra M.Brooks Annalee W.Nguyen Wen-Ling Lin Trevor G.Johnston Jennifer A.Maynard Alshakim Nelson Hal S.Alper 

机构地区：[1]Institute for Cellular and Molecular Biology,The University of Texas at Austin,Austin,TX,USA [2]McKetta Department of Chemical Engineering,The University of Texas at Austin,Austin,TX,USA [3]Department of Chemistry,University of Washington,Box 351700,Seattle,WA,USA

出　　处：《Bioactive Materials》2021年第8期2390-2399,共10页生物活性材料（英文）

基　　金：This work was supported by the Camille and Henry Dreyfus Foundation(H.A.).A.N.acknowledges both UW CoMotion and Royalty Research Fund for supporting this work.We thank Dr.Hung

摘　　要：Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity.As a result,these systems are not easily portable or reusable for on-demand protein production applications.In this study,we employ Bioproduced Proteins On Demand(Bio-POD),a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable,on-demand production and secretion of medium-and high-molecular weight proteins(in this case,SEAP,α-amylase,and anti-HER2).The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and rehydration.The hydrogel platform described here is the first hydrogel immobilization using a P.pastoris system to produce recombinant proteins of this breadth.These results highlight the potential of this formulation to establish a cost-effective bioprocessing strategy for on-demand protein production.

关 键 词：Protein production HYDROGEL Pichia pastoris IMMOBILIZATION LYOPHILIZATION 

分 类 号：R318[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]