葫芦[8]脲作为流动相添加剂的高效液相色谱法测定牛奶中喹诺酮药物  

Determination of Quinolones in Milk Using Cucurbit[8]uril as Mobile Phase Additive by High Performance Liquid Chromatography

作　　者：吴玉田 周贻兵[1] 李磊[1] 张权[1] 林野[1] 刘利亚[1] WU Yutian;ZHOU Yibing;LI Lei;ZHANG Quan;LIN Ye;LIU Liya(GuiZhou Center for Disease Control and Prevention,Guiyang 550004)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004

出　　处：《分析科学学报》2021年第3期369-372,共4页Journal of Analytical Science

基　　金：贵州省疾控中心青年基金(No.2018-E2-3)。

摘　　要：基于葫芦[8]脲能与喹诺酮分子相互作用形成稳定包结配合物,建立了以葫芦[8]脲作为流动相添加剂,测定氧氟沙星、培氟沙星、诺氟沙星、环丙沙星、沙拉沙星、西诺沙星6种喹诺酮药物的高效液相色谱法。结果显示,以含有0.05 mmol/L葫芦[8]脲的流动相进行梯度洗脱时,能够提高各喹诺酮组分之间的分离度和测定的灵敏度。该方法应用于牛奶样品检测时,在20~400 ng/mL范围内各组分线性关系良好,相关系数R^(2)均大于0.998;检出限为5~10μg/kg;精密度、稳定性良好,相对标准偏差均小于10%,加标回收率为64.0%~99.1%,满足样品测定需求。A high performance liquid chromatographic method for the determination of six quinolones including ofloxacin,pefloxacin,norfloxacin,ciprofloxacin,sarfloxacin and sinofloxacin has been established,which is based on the interaction between cucurbit[8]uril and quinolones that forms stable inclusion complexs.The results showed that the gradient elution of mobile phase containing 0.05 mmol/L cucurbit[8]uril could improve the resolution and the sensitivity.When the method was applied to the detection of six quinolones in milk samples in a range of 20-400 ng/mL,the linear relationship was good,and the correlation coefficient(R^(2))was greater than 0.998.The method showed good precision and stability,the relative standard deviation was less than 10%,the detection limit was 5-10μg/kg,and the standard recoveries were 64.0%-99.1%.The performances met the requirements of sample determination.

关 键 词：脲 喹诺酮 流动相添加剂 高效液相色谱 

分 类 号：O657.7+2[理学—分析化学]