Bio-inspired intestinal scavenger from microfluidic electrospray for detoxifying lipopolysaccharide  

作　　者：Cheng Zhao Guopu Chen Huan Wang Yuanjin Zhao Renjie Chai 

机构地区：[1]Department of Rheumatology and Immunology,Institute of Translational Medicine,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210002,China [2]State Key Laboratory of Bioelectronics,School of Life Sciences and Technology,Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research,Southeast University,Nanjing,210096,China [3]Department of Endocrinology,Shenzhen Second People’s Hospital,Center for Diabetes,Obesity and Metabolic Diseases of Shenzhen University,Health Science Center of Shenzhen University,Shenzhen,518035,PR China [4]Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,226001,China [5]Institute for Stem Cell and Regeneration,Chinese Academy of Science,Beijing,China [6]Beijing Key Laboratory of Neural Regeneration and Repair,Capital Medical University,Beijing,100069,China

出　　处：《Bioactive Materials》2021年第6期1653-1662,共10页生物活性材料（英文）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science(XDA16010303);National Key R&D Program of China(Nos.2017YFA0103903,2019YFA0111400);the National Natural Science Foundation of China(Nos.52073060,82030029 and 81970882);Natural Science Foundation from Jiangsu Province(BE2018707 and BE2019711);Shenzhen Fundamental Research Program(JCYJ20190814093401920);China Postdoctoral Science Foundation(2019M663108,2019M653061);Guangdong Basic Application Research Fund Project(2019A1515110925,2019A1515111155).

摘　　要：Lipopolysaccharide(LPS)plays an important role in metabolic syndrome(MetS)and other gut-derived diseases,and detoxifying LPS is considered to be a fundamental approach to prevent and treat these diseases.Here,inspired by the feeding behaviour of scavenger,novel microfluidic microcapsules with alkaline phosphatase(ALP)encapsulation and the scavenger-like molecular sieve shell are presented for cleaning intestinal LPS.Benefiting from the precisely controlled of the pore size and microfluidic electrospray,the generated microcapsules were imparted with porous molecular-sieve shells and ALP encapsulated active cores.These microcapsules could continuously work as an intestinal scavenger after colonized in intestine.It has been demonstrated that the microcapsules could englobe LPS while inhibit the permeation of digestive enzyme,and this ability contributes to promising ALP’s activity,protecting cells at the presence of LPS and reducing inflammation.In addition,this scavenger inspired microcapsule could effectively decrease the LPS in organs,reduce inflammation and regulating fat metabolism in vivo.These features make the ALP encapsulated microcapsules an ideal candidate for treating MetS and other LPS related diseases.

关 键 词：Biomimetic Intestinal barrier Microfluidics MICROCAPSULE Gastrointestinal disease 

分 类 号：R318[医药卫生—生物医学工程]