The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy  

作　　者：I-Hsuan Yang Yo-Shen Chen Jia-Jing Li Ya-Jyun Liang Tzu-Chieh Lin Subhaini Jakfar Minal Thacker Shinn-Chih Wu Feng-Huei Lin 

机构地区：[1]Department of Biomedical Engineering,College of Medicine and College of Engineering,National Taiwan University,No.49,Fanglan Rd,Taipei,10672,China [2]Division of Plastic Surgery,Department of Surgery,Far Eastern Memorial Hospital,No.21,Sec.2,Nanya S.Rd.,New Taipei City,220,China [3]Department of Animal Science and Technology,National Taiwan University,No.1,Section 4,Roosevelt Road,Taipei City,10617,China [4]Institute of Biomedical Engineering and Nanomedicine,National Health Research Institutes,No.35,Keyan Road,Zhunan,Miaoli County,35053,China

出　　处：《Bioactive Materials》2021年第6期1699-1710,共12页生物活性材料（英文）

基　　金：This study was supported by the National Health Research Institutes(BN-109-PP-01);National Health Research Institutes and Central Government S&T grant,Taiwan(109-1901-01-19-07);subsidized by Ministry of Science and Technology and National Taiwan University(NTU),Taiwan.

摘　　要：Many technologies have been developed for breast reconstruction after lumpectomy.Although the technologies achieved promising success in clinical,there are still many shortages hanging over and trouble the researchers.Tissue engineering technology was introduced to plastic surgery that gave a light to lumpectomy patients in breast reconstruction.The unexpected absorption rate,resulting from limited vascularization and low cell survival rate,is a major factor that leads to unsatisfactory results for the previous studies in our lab.In the study,the laminin-modified alginate synthesized by a new method of low concertation of sodium periodate would be mixed with ADSCs and Rg1 in the medium;and then sprayed into a calcium chloride(CaCl2)solution to prepare into microsphere(abbreviated as ADSC-G-LAMS)by bio-electrospray with a power syringe for the mass production and smaller bead size.The developed ADSC-G-LAMS microspheres had the diameter of 232±42μm.Sustained-release of the Rg1 retained its biological activity.WST-1,live/dead staining,and chromosome aberration assay were evaluated to confirm the safety of the microspheres.In in vivo study,ADSC-G-LAMS microspheres combined with autologous adipocytes were transplanted into the dorsum of rats by subcutaneous injection.The efficacy was investigated by H&E and immunofluorescence staining.The results showed that the bioactive ADSC-G-LAMS microspheres could integrate well into the host adipose tissue with an adequate rate of angiogenesis by constantly releasing Rg1 to enhance the ADSC or adipocyte survival rate to join tissue growth and repair with adipogenesis for breast reconstruction after lumpectomy.

关 键 词：Laminin-alginate microspheres Adipose-derived stem cells Ginsenoside Rg1 Bio-electrospray Tissue engineering 

分 类 号：R318[医药卫生—生物医学工程]