Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model  

作　　者：Xiu-Zhen Zhang Yan-Lin Jiang Jun-Gen Hu Long-Mei Zhao Qiu-Zhu Chen Yan Liang Yi Zhang Xiong-Xin Lei Rui Wang Yi Lei Qing-Yi Zhang Jesse Li-Ling Hui-Qi Xie 

机构地区：[1]Laboratory of Stem Cell and Tissue Engineering,Orthopedic Research Institute,State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University and Collaborative Innovation Center of Biotherapy,Chengdu,Sichuan,China [2]Research Core Facility of West China Hospital,Sichuan University,Chengdu,Sichuan,China

出　　处：《Bioactive Materials》2021年第6期1827-1838,共12页生物活性材料（英文）

基　　金：This work was supported by the National Key R&D Program of China(No.2017YFC1104702);Sichuan Science and Technology Program(No.2019JDRC0020);1.3.5 project for disciplines of excellence,West China Hospital,Sichuan University(No.ZYJC18002).

摘　　要：Currently the standard surgical treatment for bladder defects is augmentation cystoplasty with autologous tissues,which has many side effects.Biomaterials such as small intestine submucosa(SIS)can provide an alternative scaffold for the repair as bladder patches.Previous studies have shown that SIS could enhance the capacity and compliance of the bladder,but its application is hindered by issues like limited smooth muscle regeneration and stone formation since the fast degradation and poor mechanical properties of the SIS.Procyanidins(PC),a natural bio-crosslinking agent,has shown anti-calcification,anti-inflammatory and anti-oxidation properties.More importantly,PC and SIS can crosslink through hydrogen bonds,which may endow the material with enhanced mechanical property and stabilized functionalities.In this study,various concentrations of PC-crosslinked SIS(PC-SIS)were prepared to repair the full-thickness bladder defects,with an aim to reduce complications and enhance bladder functions.In vitro assays showed that the crosslinking has conferred the biomaterial with superior mechanical property and anti-calcification property,ability to promote smooth muscle cell adhesion and upregulate functional genes expression.Using a rabbit model with bladder defects,we demonstrated that the PC-SIS scaffold can rapidly promote in situ tissue regrowth and regeneration,in particular smooth muscle remodeling and improvement of urinary functions.The PC-SIS scaffold has therefore provided a promising material for the reconstruction of a functional bladder.

关 键 词：Bladder reconstruction Small intestinal submucosa PROCYANIDINS Bio-crosslinking Smooth muscle regeneration 

分 类 号：R318[医药卫生—生物医学工程]