B-R缓冲溶液对邻二氮菲-Fe^(2+)氧化法测定·OH的影响  

Influence of B-R Buffer Solution on Determination of·OH by 10-phenanthroline-Fe^(2+)Oxidation

作　　者：李建晴[1] 贾彦鑫 李慧颖 LI Jian-qing;JIA Yan-xin;LI Hui-ying(School of Chemical Engineering, Jinzhong University, Jinzhong 030169, China)

机构地区：[1]晋中学院化学化工系,山西晋中030619

出　　处：《运城学院学报》2021年第3期26-29,共4页Journal of Yuncheng University

基　　金：山西省自然科学基金面上项目(201901D111019);晋中学院大学生创新创业训练项目(DC202005)。

摘　　要：文章应用分光光度法对邻二氮菲-Fe^(2+)(Fe(phen)^(2+)_(3))氧化法测定羟基自由基(·OH)的影响因素进行了研究。实验利用加入抗坏血酸(Vc)对·OH的抑制作用,就反应过程中Britton-Robinson(B-R)缓冲溶液pH值、用量及试剂的加入顺序,测量Fe(phen)^(2+)_(3)在其最大吸收波长处的△A510变化值考察Vc对·OH的清除率。结果表明:不同pH值的B-R缓冲溶液,加入量和顺序不同,其清除·OH作用的效果也不同。在pH=7.54的B-R缓冲溶液用量3 mL时,Fe(phen)^(2+)_(3)体系中Vc清除·OH的效果较好。The influence factors of 10-phenanthroline-Fe^(2+)(Fe(phen)^(2+)_(3))oxidation assay of hydroxyl radical(·OH)were investigated by spectrophotometry.The inhibition of ascorbic acid(VC)on·OH was used in the experiment,and the changes of△A510 of Fe(Phen)2+3 at its maximum absorption wavelength with the pH value,the amount of Britton-Robinson(B-R)buffer solution and the addition order of reagents in the reaction process were measured to investigate the scavenging rate of VC to·OH.The results showed that in different pH value of B-R buffer solution,the effect of removing·OH was different with different addition amount and order.In pH=7.54,V=3 mL,ascorbic acid removing hydroxyl free radical effect is better.

关 键 词：B-R缓冲溶液 邻二氮菲-Fe^(2+) 羟基自由基(·OH) 

分 类 号：O657.32[理学—分析化学]