氯化镁缓释抗钙化组织工程小口径血管体外性能测试  

In vitro test of slow-release magnesium chloride of anti-calcification small caliber tissue engineering blood vessels

作　　者：李阳阳 杨英杰 刘诫 杨昭 杜鹏翀 赵亮 李温斌 Li Yangyang;Yang Yingjie;Liu Jie;Yang Zhao;Du Pengchong;Zhao liang;Li Wenbin(Department of Cardiothoracic Surgery,Third Affiliated Hospital of Xinxiang Medical College,Xinxiang 454000,China;Department of Cardiac Surgery,Beijing Institute of Cardiopulmonary Vascular Disease,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China)

机构地区：[1]河南省新乡医学院第三附属医院心胸外科,453000 [2]北京市心肺血管疾病研究所首都医科大学附属北京安贞医院心脏外科,100029

出　　处：《中华胸心血管外科杂志》2021年第5期303-308,共6页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：2019年北京市自主经费项目(201923);2018年河南省科技攻关-社会发展项目(182102310067)。

摘　　要：目的构建氯化镁(MgCl_(2))微米缓释抗钙化的组织工程小口径血管。方法联合应用Triton X-100+脱氧胆酸钠盐、DNA/RNA核酶对绵羊颈动脉行脱细胞处理,制成组织工程小口径血管支架,HE染色,电镜下观察脱细胞情况和血管支架性能。采用复乳法超声破碎高速搅拌挥发法,制备载有MgCl_(2)的微米抗钙化缓释微球颗粒。检测缓释微球的粒径、包封率、载药量(率)及测出缓释曲线。应用碳二亚胺盐酸盐/琥珀酸亚胺(EDC/NHS)交联人工小口径血管,采用冷冻干燥技术将载有MgCl_(2)的微米缓释微球与血管支架结合,电镜下观察结合情况。检测标本血管厚度、拉力强度和压力承受值。结果羊颈动脉经脱细胞处理后能去除各类细胞,并保持支架原有性能。载有MgCl_(2)的微米抗钙化缓释微球粒径(1.31±0.02)μm,相对均匀,微球颗粒包封率82.79%,载药量(率)2.98%,25天内存在缓慢释放,释放率达81.08%。载有MgCl_(2)的缓释微球颗粒与小口径组织工程血管能有效紧密结合。结论以聚乳酸-羟基乙酸共聚物为载体制成的载有MgCl_(2)的缓释微球颗粒可缓释镁离子,这为构建抗钙化组织工程小口径血管奠定了基础。Objective To construct tissue engineering small-caliber anti-calcifiction blood vessels with micron slow-release magnesium chloride.Methods After decellularizing sheep carotid artery by combining Triton X-100+deoxycholate sodium salt and DNA/RNA ribozyme,tissue engineering small-caliber vascular scaffold was made,HE staining of elastic fiber and collagen were carried out at the same time,and scanning electron microscope was used to observe the decellularization and the performance of vascular stent.The microemulsion anti-calcification slow-release microsphere particles loaded with magnesium chloride(MgCl_(2))were prepared by double emulsion method,ultrasonic breaking,high speed stirring and evaporation method.Detected the particle size,encapsulation rate,drug loading(rate)of the sustained-release microspheres and measured the sustained-release curve.After the artificial small-caliber blood vessel was cross-linked with carbodiimide hydrochloride/succinic imine(EDC/NHS),freeze-drying technology was used to combine the micron slow-release microspheres loaded with MgCl_(2) with the vascular scaffold.Observed the combination under the electron microscope,and tested the thickness and tensile strength of the specimen blood vessels.Results After decellularization,the sheep carotid artery could remove all kinds of cells and maintain the original performance of the scaffold.The averaged particle size of micro-calcium-resistant slow-release microspheres loaded with MgCl_(2) was(1.31±0.02)μm,which was relatively uniform.The encapsulation rate of microsphere particles was 82.79%,and the drug loading(rate)was 2.98%,which existed within 25 days slow release,the release rate reached 81.08%.The slow-release microsphere particles loaded with chlorinase could be effectively and tightly combined with small-caliber tissue engineering blood vessels.Conclusion The slow-release microsphere particles loaded with magnesium chloride made of PLGA as a carrier have the effect of slow-release magnesium ions.It laid the foundation for the co

关 键 词：组织工程小口径血管 抗钙化 氯化镁 缓释微球 

分 类 号：R318.08[医药卫生—生物医学工程]