西藏青稞种质资源苗期耐低氮鉴定与筛选  

Identification and Screening of Low Nitrogen Tolerance in Seedling Stage of Tibetan Highland Barley Germplasm Resources

作　　者：扎桑[1] 旺姆 徐齐君[1] Zhasang;Wangmu;XU Qi-jun(Agricultural Research InstituteTibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850032,China)

机构地区：[1]西藏自治区农牧科学院农业研究所,西藏拉萨850032

出　　处：《西藏农业科技》2021年第2期11-17,共7页Tibet Journal of Agricultural Sciences

基　　金：西藏青稞科技重大专项。

摘　　要：为选育出适应我国西部高原地区种植的耐低氮并高产的青稞品种,以1029份青稞种质资源为材料,以Hoagland营养液为介质,对2叶期的青稞幼苗进行缺氮和对照2组处理试验,通过对不同处理下青稞幼苗的株高变化量和地上生物量(鲜质量、干质量)对缺氮的响应进行分析,并通过对过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和丙二醛(MDA)活性检测对初选品种进行鉴定。结果表明:缺氮胁迫对不同青稞品种的生长影响存在显著性差异,筛选出耐低氮种质4份(“ZDM4700”“藏1446”“ZDM5713”和“ZDM5766”),不耐低氮种质4份(“藏0603”“藏0801”“ZDM5766”和“昆仑15”)。通过生长指标(株高、根长、地上生物量鲜质量和干质量,地下生物量鲜质量和干质量)和生理指标POD,CAT,APX和CAT活性的检测之后发现,30份耐贫瘠材料和30份缺氮敏感材料MDA,CAT,POD,APX含量之间无显著差异,缺氮敏感材料“ZDM5766”“ZDM5626”“ZDM4618”和“ZDM4394”缺氮培养后MDA和POD含量均高于耐贫瘠材料“ZDM4700”“ZDM4492”“ZDM5724”和“ZQ336”,说明组织遭受逆境伤害的程度大,组织中存在老化、衰弱。缺氮敏感材料CAT含量均低于耐贫瘠材料,说明组织抗氧化防御机理越低。Bare barley is called highland barley in our Tibetan area,As a staple food of the Tibetan people,It is also the main raw material for livestock feed,beer,health products and medicine.In order to select low nitrogen and high yield highland barley varieties suitable for cultivation in the western plateau of China,This study was based on 1029 barley germplasm resources,Hoagland nutrient solution,The nitrogen-deficient and control groups of highland barley seedlings in 2-leaf stage were studied,Based on the analysis of plant height variation and aboveground biomass(fresh quality,dry quality)of highland barley seedlings under different treatments,primary cultivars were identified by the detection of peroxidase(POD),apx(ascorbate peroxidase),catalase(CAT),and malondialdehyde(MDA)activities.The results showed that the effect of nitrogen deficiency stress on the growth of different highland barley varieties was significantly different,Four low-nitrogen-tolerant germplasm(ZDM4700、1446,ZDM5713、ZDM5766),4 non-tolerant low nitrogen germplasm(Tibetan 0603,Tibetan 0801,ZDM5766、Kunlun 15).Through growth index(plant height,root length,fresh dry weight of aboveground biomass,The aboveground biomass fresh dry weight)and physiological index POD、CAT、APX and CAT activity were detected,However,there was no significant difference between the MDA、CAT、POD、APX content of 30 refractory materials and 30 nitrogen-deficient sensitive materials,The content of nitrogen-deficient sensitive materials ZDM5766、ZDM5626、ZDM4618、ZDM4394 nitrogen-deficient culture MDA、POD higher than that of poor resistant materials Showing the extent to which the organization suffers from adversity,There is aging and weakness in the tissue.CAT content of nitrogen-deficient sensitive materials was lower than that of barren resistant materials,indicating that the mechanism of tissue antioxidant defense was lower.A significant difference in APX content.

关 键 词：青稞幼苗 耐低氮性 筛选 

分 类 号：S503.4[农业科学—作物学]