参芪复方对2型糖尿病模型大鼠骨骼肌病变的影响  

Effect of Shenqi Compound Formula(参芪复方)on Skeletal Muscle Lesions in Rats Model of Type 2 Diabetes Mellitus

作　　者：张海燕 吴贤波 丁若兰[3] 朱海燕 赵昱东 ZHANG Haiyan;WU Xianbo;DING Ruolan;ZHU Haiyan;ZHAO Yudong(Clinical Medical College of Chengdu University of Traditional Chinese Medicine,Chengdu,610075;Chengdu Sport University;Affiliated Hospital of Chengdu University of Traditional Chinese Medicine;The First Affiliated Hospital of Chengdu Medical College)

机构地区：[1]成都中医药大学临床医学院,四川成都610075 [2]成都体育学院 [3]成都中医药大学附属医院 [4]成都医学院临床医学院第一附属医院

出　　处：《中医杂志》2021年第12期1080-1085,共6页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81660788);国家中医药管理局应用基础项目(yyjc20200907);四川省中医药管理局科学技术研究专项(2018QN051);成都医学院第一附属医院高层次人才科研启动基金(CYFY-GQ02)。

摘　　要：目的探讨参芪复方防治2型糖尿病(T2DM)骨骼肌病变的可能作用机制。方法自发性2型糖尿病GK大鼠联合高脂饲料喂养制备T2DM大鼠模型。造模成功后,30只GK大鼠随机分为模型组、参芪复方组、罗格列酮组,每组10只,另设10只Wistar大鼠作为正常对照组。参芪复方组给予参芪复方浸膏14.4g/(kg·d)灌胃,罗格列酮组给予罗格列酮混悬液0.67 mg/(kg·d)灌胃,模型组和正常对照组均给予等体积生理盐水灌胃,均每日1次。8周后观察大鼠骨骼肌线粒体病理学改变;检测各组大鼠骨骼肌线粒体的线粒体融合蛋白1(Mfn1)、线粒体融合蛋白2(Mfn2)、视神经萎缩因子1(Opa1)、动力相关蛋白1(Drp1)、线粒体分裂蛋白1(Fisl)、线粒体分裂因子(Mff)蛋白及mRNA表达。结果与正常对照组比较,模型组Mfn1、Mfn2蛋白及mRNA表达减少,Drp1蛋白及mRNA表达增多(P<0.05);与模型组比较,参芪复方组Mfn1、Mfn2蛋白及mRNA表达增多,Drp1蛋白及mRNA表达减少(P<0.05)。参芪复方组与罗格列酮组Mfn1、Mfn2、Opa1、Drp1、Fisl、Mff蛋白及mRNA表达差异均无统计学意义(P>0.05)。病理学结果显示,模型组大鼠骨骼肌线粒体肿胀、嵴结构不清,或见絮状变性及空泡状改变;参芪复方组大鼠骨骼肌线粒体仅轻度肿胀,肌纤维排列整齐,无萎缩;罗格列酮组可见骨骼肌线粒体肿胀、变性或见空泡形成,嵴走向紊乱、模糊。结论参芪复方可以明显改善T2DM模型大鼠骨骼肌早期病变,其作用机制可能与影响线粒体动力学密切相关。Objective To explore the potential mechanism of Shenqi Compound Formula(SCF,参芪复方)on skeletal muscle lesions in rats model of type 2 diabetes mellitus(T2 DM).Methods Thirty GK rats with spontaneous T2 DM were fed with high-fat diet to develop the T2 DM rat model.After success of modeling,30 GK rats were randomly divided into model group,SCF group and rosiglitazone group,with 10 rats in each group;additionally,10 Wistar rats were given normal feed as control group.SCF group was administrated with 14.4 g/(kg·d)Shenqi Compound extract;rosiglitazone group was administrated with 0.67 mg/(kg·d)rosiglitazone suspension;model group and control group were given equal volume of normal saline;the interventions in all groups were administered once daily.After eight weeks,the pathological changes of skeletal muscle mitochondria were assessed;the protein and mRNA expressions of mitochondrial fusion protein 1(Mfn1),mitochondrial fusion protein 2(Mfn2),optic atrophy factor 1(Opa1),dynamic related protein 1(Drp1),mitochondrial fission protein 1(Fis1),mitochondrial fission factor(Mff)in the skeletal muscle mitochondria of rats were detected.Results Compared to the control group,the model group had decreased expression of Mfn1,Mfn2 protein and mRNA,and increased expression of Drp1 protein and mRNA(P<0.05).Compared to the model group,the SCF group had higher expression of Mfn1,Mfn2 protein and mRNA,and lower expression of Drp1 protein and mRNA(P<0.05).There were no significant differences in the protein and mRNA expressions of Mfn1,Mfn2,Opa1,Drp1,Fis1 and Mff between the SCF group and the rosiglitazone group(P>0.05).The pathological results showed that in model group,skeletal muscle mitochondria were swollen;the cristae structure was unclear,or flocculent degeneration and vacuolar changes were observed.In SCF group,skeletal muscle mitochondria were slightly swollen;muscle fibers were neatly arranged without atrophy.In rosiglitazone group,skeletal muscle mitochondria were swollen and degenerated;vacuoles were formulated;and

关 键 词：2型糖尿病 骨骼肌 参芪复方 线粒体 线粒体动力学 

分 类 号：R285.5[医药卫生—中药学]