竹节参总皂苷大孔树脂纯化工艺研究  

Study on purification technic of total saponins in Panacis Japonici Rhizoma with macroporous resin

作　　者：王晶 董一鑫 贺丽莹 苏比努尔·亚森 孙逸轩 于萍[1,2] 邹海艳 Wang Jing;Dong Yixin;He Liying;Subinuer Yasen;Sun Yixuan;Yu Ping;Zou Haiyan(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Lab of TCM Collateral Disease Theory Research,Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中医络病研究北京市重点实验室,北京100069

出　　处：《国际中医中药杂志》2021年第6期570-574,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81403095、81973564);北京市教委科技计划资助项目(KM201910025020);北京高等学校高水平人才交叉培养大学生科研训练计划深化项目(2021年)。

摘　　要：目的筛选适合纯化竹节参总皂苷的大孔树脂,并确定其纯化工艺参数。方法以总皂苷含量为指标,采用静态吸附试验,结合吸附动力学参数筛选大孔树脂类型;通过动态吸附试验考察竹节参提取物大孔树脂纯化的工艺参数,确定竹节参总皂苷的制备工艺。结果D101大孔树脂对竹节参总皂苷有较好的吸附和解吸附效果,其最佳纯化工艺参数为:上样质量浓度为0.1 g/ml(以生药计),上样体积为100 ml,即树脂上样量相当于3.3 g生药/ml;洗脱时先以3倍柱体积的蒸馏水、20%乙醇除杂,再用6倍柱体积的70%乙醇洗脱富集总皂苷,上样和洗脱流速为0.5 ml/min,总皂苷转移率可达85.6%。结论D101大孔树脂可有效富集和纯化竹节参总皂苷,可为竹节参药材的开发和利用提供依据。Objective Select a suitable macroporous resin for the purification technic of total saponins from Panacis Japonici Rhizoma and determine the parameter of purification technic.Methods Made the content of total saponins as the index,used static adsorption test and combined the adsorption kinetic parameters to select the type of macroporous resin.By using dynamic adsorption experiment to investigate the technical parameters of the purified macroporous resin extracted from Panacis Japonici Rhizoma.Then the preparation technic of the total saponins of Panacis Japonici Rhizoma was determined.Results The D101 macroporous resin could absorpt and desorpt total saponins of Panacis Japonici Rhizoma effectively.The optimal purification parameters were as follow:the loading mass concentration was 0.1 g/ml(based on crude drug),and the loading volume was 100 ml(which means the loading volume of resin per ml was equivalent to 3.3 grams of crude drug).During the elution process,distilled water(3 BV)and 20%ethanol(3 BV)were used to remove impurity,and then 70%ethanol elution(6 BV)was used to enrich the total saponins.The flow rate of loading and elution was 0.5 ml/min.The transfer rate of total saponins could reache 85.6%.Conclusion The D101 macroporous resin can effectively enrich and purify the total saponins of Panacis Japonici Rhizoma,which provides the scientific basis for the development and utilization of Panacis Japonici Rhizoma.

关 键 词：竹节参 皂苷类 大孔树脂 吸附动力学 中药工艺改进 

分 类 号：R284.2[医药卫生—中药学]