基于网络药理学探讨固肠止泻丸干预溃疡性结肠炎分子机制  

To explore the molecular mechanism of Guchang-Zhixie pill in the treatment of ulcerative colitis based on network pharmacology

作　　者：许建秦 刘改霞 张涛 李耀辉[1] Xu Jianqin;Liu Gaixia;Zhang Tao;Li Yaohui(Department of Spleen and Stomach,Shaanxi Traditional Chinese Medicine Hospital,Xi’an 71000,China;Department of Traditional Chinese Medicine,the First Clinical Medical College,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,China;Department of Pulmonary Disease,Ankang Traditional Chinese Medicine Hospital of Shaanxi,Ankang 725000,China)

机构地区：[1]陕西省中医医院脾胃科,西安710000 [2]陕西中医药大学第一临床医学院中医系,咸阳712046 [3]陕西省安康市中医医院肺病科,725000

出　　处：《国际中医中药杂志》2021年第6期588-593,共6页International Journal of Traditional Chinese Medicine

基　　金：陕西省科技厅重点研发计划(2020SF-351)。

摘　　要：目的基于网络药理学探讨固肠止泻丸治疗溃疡性结肠炎的分子机制。方法检索TCMSP数据库得到固肠止泻丸中各药物的有效成分及靶基因;检索在线人类孟德尔遗传数据库(OMIM)、DisGeNET数据库获得溃疡性结肠炎的基因,将药物基因与疾病基因取交集得到核心基因;采用STRING数据库构建基因功能关联网络,采用DAVID数据库对核心基因进行GO富集分析和通路富集分析,并采用OmicShare平台绘制气泡图,Cytoscape软件绘制“靶点-GO-通路”网络。结果共获得固肠止泻丸有效成分77种,靶点211个;获得溃疡性结肠炎914个基因;将药物基因与疾病基因取交集共得到72个核心基因,拓扑分析显示,核心靶点为IL6、IL1B、MAPK1、VEGFA、MMP9等;共得到12个富集的生物过程聚类,联系靶点较多的生物过程有RNA聚合酶Ⅱ启动子转录的正调控、以DNA为模板的转录正调节;通路富集分析共获得14个富集的通路聚类,其中与炎症密切相关并且联系靶点较多的通路有TNF信号通路和PI3K/Akt信号通路。结论本研究通过数据库分析得到了固肠止泻丸干预溃疡性结肠炎的作用靶点及作用通路,为明确其作用机制提供参考。Objective To explore the molecular mechanism of Guchang-Zhixie pill in the treatment of ulcerative colitis based on network pharmacology.Methods Retrieve the TCMSP database to get the effective components and target genes of each drug in Guchang-Zhixie pill,and then retrieve the OMIM database Disgenet database to get disease genes,and then intersect the drug genes and disease genes to get the core genes.Used STRING database to build gene function association network,and used DAVID database to analize go enrichment and pathway enrichment of the core genes.Results A total of 77 active ingredients and 211 targets of Guchang-Zhixie pill were obtained by TCMSP database,914 genes of ulcerative colitis were obtained by retrieving disease gene database,72 core genes were obtained by intersection of drug gene and disease gene.Topology analysis showed that the core targets were IL6,IL1B,MAPK1,VEGFA,MMP9,etc;Twelve enriched biological process clusters were obtained.The biological processes with more contact targets were positive regulation of RNA polymeraseⅡpromoter transcription and positive regulation of DNA template transcription;A total of 14 enriched pathway clusters were obtained by pathway enrichment analysis,among which TNF signaling pathway and PI3K/Akt signaling pathway were closely related to inflammation and associated with more targets.Conclusion The target and pathway of Guchang-Zhixie pill in the treatment of ulcerative colitis are preliminarily obtained through database analysis,which has provided the reference for clarifying its mechanism.

关 键 词：结肠炎 溃疡性 固肠止泻丸 网络药理学 药理作用分子作用机制(中药) 

分 类 号：R285[医药卫生—中药学]