一测多评法测定藏族药烈香杜鹃中5种黄酮类成分的含量  

Content determination of five flavonoids in Tibetan medicine Rhododendron anthopogonoides by quantitative analysis of multi-components by single marker(QAMS)

作　　者：彭瑶 曾嵘[1] 吴尚洁[2] 雷思[2] 孙智娜[3] 何秀琴[3] 李娟[1] 李顺祥[1] PENG Yao;ZENG Rong;WU Shang-Jie;LEI Si;SUN Zhi-na;HE Xiu-qin;LI Juan;LI Shun-xiang(Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine,School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;the Second Xiangya Hospital of Central South University,Changsha 410008,China;Qinghai Red Cross Hospital,Xining 810000,China)

机构地区：[1]湖南中医药大学药学院中药活性物质筛选工程技术研究中心,湖南长沙410208 [2]中南大学湘雅二医院,湖南长沙410008 [3]青海红十字医院,青海西宁810000

出　　处：《中国中药杂志》2021年第9期2229-2236,共8页China Journal of Chinese Materia Medica

基　　金：青海省科技成果转化专项(2017SF122);中南大学新型冠状病毒感染肺炎防控应急项目(160260005);湖南中医药大学中药学一流学科项目(校行科字[2018]3号)。

摘　　要：建立烈香杜鹃中5种黄酮类成分的一测多评法,并验证其在烈香杜鹃药材中的可行性和适用性。采用高效液相色谱法(HPLC),以金丝桃苷为内参物,建立芦丁、槲皮苷、槲皮素和山柰酚的相对校正因子(RCF),计算烈香杜鹃样品中其他4种黄酮类成分的质量分数,同时考察其系统耐用性和色谱峰的定位,采用一测多评法(QAMS)和外标法(ESM)分别测定12批来源不同产地的烈香杜鹃药材中5种黄酮类成分的质量分数,并对2种测定方法所得结果进行统计分析,以验证一测多评法的准确性及可行性。结合烈香杜鹃指纹图谱和聚类分析数据,就药材批次间的差异性进行分析及讨论。结果表明,芦丁、槲皮苷、槲皮素和山柰酚的相对校正因子分别为1.2426,0.9905,0.5350和0.7813,在不同实验条件下相对校正因子重现性良好,采用一测多评法和外标法测得12批样品含量测定结果无显著差异,指纹图谱和聚类分析数据表明分类情况与该药材的产地分布情况相一致。这表明建立以金丝桃苷为内参物,同时测定芦丁、槲皮苷、槲皮素和山柰酚的一测多评法是准确且可靠的,可用于烈香杜鹃药材的质量控制。To establish a quantitative analysis of multi-components by single marker(QAMS)method for five flavonoids in Rhododendron anthopogonoides and verify its feasibility and applicability in the medicinal materials of R.anthopogonoides.With hyperoside as the internal reference,relative correction factors(RCF)of rutin,quercetin,quercitrin and kaempferol were established by high-performance liquid chromatography(HPLC)analysis.RCFs were used to calculate the content of each component,system durability and relative retention time.Simultaneously,QAMS and external standard method(ESM)were used to determine the content of five flavonoids in 12 batches of R.anthopogonoides from different origins.The results were statistically analyzed to verify the accuracy and feasibility.The fingerprints and cluster analysis data of R.anthopogonoides analyzed and discussed differences among the batches.According to the results,the RCFs of rutin,quercetin,quercetin and kaempferol in R.anthopogonoides were 1.2426,0.9905,0.5350,and 0.7813,respectively.The RCFs represented a good reproducibility under different experimental conditions.Besides,there was no significant difference between QAMS and ESM.Besides,the fingerprint and cluster analysis data showed the consistency between the classification and with the origin distribution of the herbs.In conclusion,the QAMS method shows a good stability and accuracy in the quality control of R.anthopogonoides.

关 键 词：烈香杜鹃 一测多评 相对校正因子 金丝桃苷 芦丁 

分 类 号：R284.1[医药卫生—中药学]