欧洲花楸悬浮细胞对生物胁迫的记忆功能初探  

Preliminary study on memory function of Sorbus aucuparia suspension cell to biotic stress

作　　者：袁杰 王升[2] 刘亚辉 李佳兴 周良云[2] 李锬 周利[2] 张文晋 郭兰萍[2] 黄璐琦 YUAN Jie;WANG Sheng;LIU Ya-hui;LI Jia-xing;ZHOU Liang-yun;LI Tan;ZHOU Li;ZHANG Wen-jin;GUO Lan-ping;HUANG Lu-qi(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [3]中国中医科学院,北京100700

出　　处：《中国中药杂志》2021年第10期2467-2473,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81703648,81891014);国家自然科学基金项目(81703655);中央级公益性科研院所基本科研业务费专项(ZZXT201901,ZZ13-YQ-084);国家重点研发计划项目(2017YFC1700701,2017YFC1701405)。

摘　　要：植物对其所遭受的环境胁迫存在记忆功能,当再次受到重复的环境胁迫时能更快更好地启动对环境胁迫的应答和适应机制,从而实现长期稳定的繁衍生存。但是这类研究大都体现在农作物以及模式植物拟南芥中,在药用植物中少有研究这种记忆功能对植物生长状态的作用以及对植保素生物合成的影响。该研究采用酵母提取物(YE)作为模拟生物胁迫的诱导子,通过对欧洲花楸悬浮细胞给予重复生物胁迫,测定了重复胁迫及单次胁迫下生物量以及部分次生代谢产物含量的变化。结果表明,与第一次受到环境胁迫的欧洲花楸悬浮细胞相比,受到重复胁迫的欧洲花楸悬浮细胞在再次受到环境胁迫后生物量积累水平在部分时间点下显著提高,次生代谢产物在部分时间点下积累水平也显著升高。上述工作确定了欧洲花楸悬浮细胞存在记忆环境胁迫的现象,以及这种记忆环境胁迫的现象对欧洲花楸悬浮细胞部分次生代谢产物合成与积累的影响,为进一步研究药用植物的胁迫记忆功能及其代际遗传机制奠定了基础。Plants have a memory function for the environmental stress they have suffered.When they are subjected to repeated environmental stress,they can quickly and better activate the response and adaptation mechanism to environmental stress,thus realizing long-term stable reproduction.However,most of the relevant studies are applied to crops and Arabidopsis thaliana rather than medicinal plants about the improvement of plant growth status and the effect on phytoalexin biosynthesis.In this study,yeast extract(YE)was used as an elicitor to simulate biotic stress,and the changes in biomass and the content of some secondary metabolites were measured by giving repeated stresses to Sorbus aucuparia suspension cell(SASC).The results showed that the accumulation levels of biomass and some secondary metabolites in SASC subjected to repeated stress are significantly increased at some time points compared with single stress.A phenomenon that SASC can memorize biotic stress is confirmed in this study and influences phytoalexin accumulation in SASC.Furthermore,the work laid the groundwork for research into the transgenerational stress memory mechanism of medicinal plant.

关 键 词：欧洲花楸 悬浮细胞 生物胁迫 重复胁迫 胁迫记忆 

分 类 号：S567.19[农业科学—中草药栽培]