基于指纹图谱段带总量统计矩法和信息熵的桑源药材成分比较分析研究  

Comparative analysis and research on composition of mulberry-sourced medicinal materials by fingerprint division total statistical moment method and information entropy

作　　者：朱志飞 刘金玲[1,2,3] 樊启猛 刘有志 吴月峰[1,2,3] 周晋 贺福元 ZHU Zhi-fei;LIU Jin-ling;FAN Qi-meng;LIU You-zhi;WU Yue-feng;ZHOU Jin;HE Fu-yuan(College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory of Drug Gability and Preparation Modification of Traditional Chinese Medicine,Changsha 410208,China;Laboratory of Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medica,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208 [3]湖南中医药大学中医药超分子机理与数理特征化实验室,湖南长沙410208

出　　处：《中国中药杂志》2021年第10期2547-2555,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81903759,81874507);湖南省教育厅项目(18K071);湖南省药学一流学科开放基金项目(2018XY09);中药成药性与制剂制备湖南省重点实验室开放基金项目(2017);国家留学基金项目(201708430243);长沙市科学技术局项目(kq2004059);研究生创新课题(2018CX56)。

摘　　要：运用段带总量统计矩法和信息熵对桑枝、桑叶、桑白皮及桑椹4种桑源药材HPLC指纹图谱进行分析,研究桑不同入药部位化学成分及其印迹模板整体特征的差异,为寻找桑源药材"同源异效"药效物质提供基础。建立桑源药材桑枝、桑叶、桑白皮及桑椹各6批次的指纹图谱,通过计算其段带总量统计矩参数和信息熵,对桑源药材指纹图谱进行异同分析。桑源药材桑枝、桑叶、桑白皮及桑椹指纹图谱AUCT,MCRTT,VCRTT,H成对配对t检验P均小于0.05,具有显著性差异。其中,4味桑源药材均在3~11,20~24 min同时有吸收峰,说明4味桑源药材在上述相同时间段有相同或相似的化学成分,在34 min后,4味桑源药材均无吸收峰出现。4味桑源药材指纹图谱在保留时间3~4,16~18,25~27,31~32 min时VCRTT均较大,说明4味桑源药材间的成分在这些时间段内存在显著差异;且3~4,15~17 min,桑椹VCRTT明显高于其余3味药材;在8~10,28~30 min,桑白皮的VCRTT显著高于其余3味药材;21~23 min,4味桑源药材的VCRTT均较低,说明4味桑源药材在这一时间段色谱图内含有相同或相似的成分,但含量与配比可能存在差异。此外,桑枝在7~12,23~27 min处信息熵最大,桑椹在2~8 min处信息熵最大,说明桑枝、桑椹在相应时间段内成分响应较大,也是其化学成分的主要出峰时间段,对应此处的化学成分及相应药效。结果表明,桑源药材间成分和含量具有显著差异性,段带总量统计矩和信息熵可用于"同源异效"中药成分差异变化分析,为其确定质量标志物提供更为全面的分析方法。The present work is to analyze the HPLC fingerprints of mulberry-sourced materials(Mori Ramulus,Mori Folium,Muri Cortex,Mori Fructus)using the fingerprint division total statistical moment method and information entropy,and to study the diffe-rences of the chemical components and the overall characteristics of the imprinting template in different parts of mulberry-sourced medicinal materials,so as to provide the basis for finding the effective substances in response to"homologous and different effect"of mulberry(Morus alba).The fingerprints of 24 batches of mulberry-related materials,such as Mori Ramulus,Mori Folium,Muri Cortex,Mori Fructus,were established,and the similarities and differences of the fingerprints were analyzed by calculating the division total statistical moment parameters and information entropy.The AUCT,MCRTT,VCRTT and H values of 24 batches of mulberry-sourced materials were less than 0.05,with significant difference.Among them,all samples showed absorption peaks within 3-11,and 20-24 min,indicating that the four types had the identical or similar chemical composition in the same time period.After 34 min,none of the four types showed absorption peaks.Greater VCRTT value of the fingerprints of the four kinds was observed at the retention time ranges of 3-4,16-18,25-27,and 31-32 min,indicating that the components of the four kinds were significantly different in these time periods;and VCRTT value of the mulberry was significantly higher than that of the other three kinds of medicinal materials at the retention time windows of 3-4 and 15-17 min;the VCRTT value of the mulberry white skin was significantly higher at the time windows of 8-10 and 28-30 min;the VCRTT value of all four kinds was significantly higher within 21-23 min,indicating that the four herbs contain the same or similar components in the chromatogram during this period,but there may be significant differences between the content and the proportion.In addition,the information entropy of mulberry branches is the largest at 7-12,23-27

关 键 词：桑源药材 同源异效 超分子“印迹模板” 指纹图谱 段带 总量统计矩 信息熵 HPLC 

分 类 号：R284.1[医药卫生—中药学]