基于组效关系的石斛抑制胃癌细胞生长的活性成分辨识  

Identification of active components of Dendrobium inhibit growth of gastric cancer cells based on component-activity relationship

作　　者：刘佳妮 王凤忠[1] 刘佳萌[1] 贾宁 孙玉凤[1] 王慧 孙晶 范蓓[1] LIU Jia-ni;WANG Feng-zhong;LIU Jia-meng;JIA Ning;SUN Yu-feng;WANG Hui;SUN Jing;FAN Bei(Key Laboratory of Agro-Products Quality and Safety in Harvest,Storage,Transportation,Management and Control,Ministry of Agriculture and Rual Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院农产品加工研究所农业农村部农产品质量安全收贮运管控重点实验室,北京100193

出　　处：《中国中药杂志》2021年第10期2565-2570,共6页China Journal of Chinese Materia Medica

基　　金：现代农业产业技术体系建设专项(CARS-21);中央级公益性科研院所基本科研业务费专项(Y2020XK24,Y-2017PT43-01,Y2020PT33)。

摘　　要：采用SGC-7901,HGC-27,MGC-8033种胃癌细胞系,对7个不同品种石斛抑制胃癌细胞生长的作用进行了活性测试;同时,采用UPLC-Q-TOF-MS将7种石斛提取物进行了化学成分表征,发现了504个共有峰。基于不同成分对活性的贡献不同,采用灰色关联度(GRA)与偏最小二乘法(PLS)2种统计分析方法建立"组效关系",辨识潜在的活性成分,利用课题组前期建立的石斛属植物化学成分数据库、对照品比对以及Nature Chemistry,MassBank等数据库搜索,辨识出4个抑制胃癌细胞生长的活性成分,分别为3,5,9-trihydroxy-23-methylergosta-7,22-dien-6-one、diacylglycerol(14∶1/22∶6/0∶0)、pipercitine和22-tricosenoic acid。Three cancer cell lines including gastric cancer SGC-7901,HGC-27,and MGC-803 cells were employed to evaluate the bioactivity of seven Dendrobium species.Simultaneously,these Dendrobium species were assessed with UPLC-Q-TOF-MS,and 504 common peaks were found.Based on the hypothesis that biological effects varied with differences in components,multivariate relevance analysis for chemical component-activity relationship of Dendrobium,including grey relation(GRA)and partial least squares(PLS)analysis were performed to evaluate the contribution of each identified component.The target peaks were identified by standards toge-ther with databases of Dendrobium,Nature Chemistry,MassBank,etc.Finally,four active components,including 3,5,9-trihydroxy-23-methylergosta-7,22-dien-6-one,diacylglycerol(14∶1/22∶6/0∶0),pipercitine,and 22-tricosenoic acid,might have negative effect on the growth of gastric cancer cells.

关 键 词：石斛 组效关系 胃癌 灰色关联度 偏最小二乘回归方法 

分 类 号：R285[医药卫生—中药学]