Polyphyly and species delimitation of Picea brachytyla(Pinaceae)based on population genetic data  

作　　者：Le-Ke Lyu Dong-Lei Wang Long Li Ying-Ying Zhu De-Chun Jiang Jian-Quan Liu Xiao-Ting Xu 

机构地区：[1]Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China [2]State Key Laboratory of Grassland Agro-Ecosystem,Institute of Innovation Ecology and College of Life Sciences,Lanzhou University,Lanzhou 730000,China

出　　处：《Journal of Systematics and Evolution》2021年第3期515-523,共9页植物分类学报（英文版）

基　　金：This work was supported by grants from National Key Research and Development Program(2017YFC0505203);National Natural Science Foundation of China(31590821,31700323,and 41771055);Fundamental Research Funds for the Central Universities(2018CDDY-S02-SCU,SCU2019D013,and YJ201721).

摘　　要：Accurate species delimitation is fundamental to biodiversity conservation.The endangered spruce Picea brachytyla(Franch.)E.Pritz.was suggested to be polyphyletic based on a limited number of samples in previous studies.To evaluate polyphyly of P.brachytyla,we sampled 139 individuals from 16 populations across most of its distributional range,plus representatives of two related species,Picea likiangensis(Franch.)E.Pritz.and Picea wilsonii Mast.We sequenced 13 nuclear loci and three chloroplast and two mitochondrial loci for the following species delimitation.Phylogenetic analyses of nuclear loci grouped all individuals of P.brachytyla from Sichuan and Chongqing into one distinct lineage and those from Yunnan and Tibet(southern distribution)nested within the P.likiangensis species complex.Structure analyses confirmed this result.Networks of chloroplast DNA haplotypes similarly showed that P.brachytyla from the southern distribution nested within the P.likiangensis species complex,whereas haplotypes for the northern distribution comprised a separate and well-supported lineage.These results suggest that P.brachytyla from the southern distribution is a part of the P.likiangensis species complex and should be removed from P.brachytyla.Our study highlights the utility of population genetic evidence in delimitating endangered species and understanding the conservation status of such species.

关 键 词：Picea brachytyla POLYPHYLY population genetic data species delimitation 

分 类 号：Q94[生物学—植物学]