铜绿假单胞菌必需基因的研究进展  

Progress of essential genes in Pseudomonas aeruginosa

作　　者：张明月 胡福泉[2] 黄广涛 ZHANG Mingyue;HU Fuquan;HUANG Guangtao(The First Clinical Institute,Zunyi Medical University,Zunyi,Guizhou 563000,China;Department of Microbiology,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing 400038,China;Department of Burn Plastic Surgery,Affiliated Hospital of Zunyi Medical University,Guizhou Clinical(Wound Healing)Research Center,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医科大学第一临床学院,贵州遵义563000 [2]陆军军医大学(第三军医大学)基础医学院微生物学教研室,重庆400038 [3]遵义医科大学附属医院烧伤整形外科贵州省临床(创面修复)研究中心,贵州遵义563000

出　　处：《微生物学通报》2021年第6期2143-2154,共12页Microbiology China

基　　金：国家自然科学基金(81801916,81960353);贵州省科技计划(2020-4Y003,2020-1Y332);遵义市科技局联合基金(HZ-2019-50)。

摘　　要：铜绿假单胞菌为专性需氧非发酵革兰氏阴性杆菌,是医院感染的常见条件致病菌之一,可引起呼吸道、泌尿道、烧伤创面和菌血症等严重感染。铜绿假单胞菌耐药形势日益严峻,给临床治疗带来困难。必需基因是生长过程中必不可少的看家基因,对铜绿假单胞菌必需基因进行深入研究,不仅有助于了解细菌的生长、毒力等基本特性,也有助于筛选新的抗菌药物靶标。本文针对铜绿假单胞菌及其必需基因进行综述,首先介绍了铜绿假单胞菌的基本生理特性及目前耐药趋势,又归纳了必需基因的研究方法,最后对铜绿假单胞菌必需基因的研究进展进行总结。Pseudomonas aeruginosa(P. aeruginosa), an obligatory aerobic non-fermented Gram-negative bacterium, is one of the common opportunistic pathogens of nosocomial infection. P. aeruginosa can cause severe infection such as respiratory tract, urinary tract, burn wounds and bacteremia. The antibiotic resistance of P. aeruginosa spread widely, which brings difficulties to clinical treatment. Essential genes are indispensable genes for organisms to grow. Investigation of P. aeruginosa essential genes could help us to understand the basic characteristics of bacteria such as growth and virulence, and could also help us to screen new targets of antibacterial agents. This paper will summary the progress of P. aeruginosa essential genes, including basic physiological characteristics of P. aeruginosa, the research methods of essential genes and the progress and significance of P. aeruginosa essential genes.

关 键 词：铜绿假单胞菌 必需基因 转座子 生物信息学 CRISPRi 

分 类 号：R378.991[医药卫生—病原生物学]