一种外周血染色体制片技术的效果评价  

Evaluation of a technique for preparing peripheral blood chromosomes

作　　者：谢节 杨素霞 常中宝 卞林翠 XIE Jie;YANG Suxia;CHANG Zhongbao;BIAN Lincui(Cell Genetics Laboratory of Hefei Jinyu Medical Laboratory Co.,Ltd.,Hefei,Anhui 231000,China)

机构地区：[1]合肥金域医学检验实验室有限公司细胞遗传室,安徽合肥231000

出　　处：《中国优生与遗传杂志》2021年第2期255-257,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨一种简单稳定的外周血染色体的滴片方法,从而提高染色体的制片和分析效率。方法使用本实验室正常收获制备的细胞悬液,采用2种不同滴片方法进行滴片,观察并分析不同滴片方法制备的染色体的分散面积、带纹质量以及批间稳定性。结果采用常温水膜分散法获得的分裂相分布均匀,分散面积大,带纹质量高,批间稳定性高。条带水平达到400带及以上的占比63.5%,高于传统冰片法(41.0%)(P<0.01)。结论通过本实验研究发现了一种简单稳定的染色体制片技术,提高了染色体的分析效率,可在各个实验室推广使用。Objective To explore a simple and stable method of dropping chromosomes in peripheral blood to improve the efficiency of chromosome preparation and analysis.Methods Using the cell suspension prepared by normal harvest in this room,the dispersion area,band quality and inter-batch stability of prepared chromosomes were observed and analyzed by two different dropping methods.Results The split phase obtained by water film dispersion method at room temperature was uniformly distributed,with large dispersion area,high stripe quality and high inter-batch stability.The band level of 400 bands and above accounted for 63.5%,which was higher than the traditional borneol method(41.0%)(P<0.01).Conclusion A simple and stable chromosome preparing technique was found,which improved the efficiency of chromosome analysis and could be popularized in various laboratories.

关 键 词：染色体分散 常温 冰片 水膜 

分 类 号：R446.8[医药卫生—诊断学]