慢性高尿酸血症肾损害大鼠模型的建立  

Establishment of a rat model of chronic hyperuricemia with renal damage

作　　者：刘冬恋 郭秋鸿 夏阳淼 周叶 李佳 LIU Donglian;GUO Qiuhong;XIA Yangmiao;ZHOU Ye;LI Jia(Collaborative Innovation Center of Sichuan for Elderly Care and Health,Chengdu Medical College,Chengdu 610500,China;Medical Records and Statistics Department of 363 Hospital,Chengdu 610041)

机构地区：[1]成都医学院四川养老与老年健康协同创新中心,成都610500 [2]三六三医院病案与统计科,成都610041

出　　处：《中国实验动物学报》2021年第3期364-370,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：成都医学院四川养老与老年健康协同创新中心资助项目(19Z08);大学生创新创业训练计划项目(S201913705037)。

摘　　要：目的通过建立慢性高尿酸血症肾损害大鼠模型,为抗慢性高尿酸血症肾损害的药物研发提供模型工具。方法雄性SD大鼠40只,随机分为5组:正常组、A组(2%氧嗪酸钾+12%酵母膏+86%普通饲料饲喂)、B组(0.15%腺嘌呤+10%酵母膏+89.85%普通饲料饲喂)、C组(100 mg/kg腺嘌呤+1500 mg/kg氧嗪酸钾,每日早晚各灌胃1次)和D组(50 mg/kg腺嘌呤+1500 mg/kg氧嗪酸钾,每日早上灌胃1次)。观察时间为5周,每周检测各组大鼠体重、大鼠血清尿酸、肌酐、尿素氮等指标,5周后,检测大鼠双肾重与体重的比值,同时对肾进行病理切片观察。结果与正常组相比,C组大鼠体重减轻,双肾重与体重的比值升高,血清尿酸、肌酐和尿素氮均升高,肾颜色变化明显,HE和尿酸盐染色发现肾有一定程度的损害。结论100 mg/kg腺嘌呤+1500 mg/kg氧嗪酸钾,每日早晚灌胃的方式建立慢性高尿酸血症肾损害大鼠模型的效果最佳。Objective To establish a rat model of chronic hyperuricemia renal damage,and to provide a model tool for the development of drugs for anti-chronic hyperuricemia renal damage.Methods Forty male sprague-dawley(SD)rats were randomly divided into five groups:normal group,group A(fed with 2%oteracil potassium+12%yeast extract+86%common feed),group B(fed with 0.15%adenine+10%yeast extract+89.85%common feed),group C(100 mg/kg adenine+1500 mg/kg oteracil potassium,intragastric administration in the morning and evening),and group D(50 mg/kg adenine+1500 mg/kg oteracil potassium,intragastric administration every morning).The observation time was5 weeks.The body weight,serum uric acid,creatinine,urea nitrogen,and other indicators of rats in each group were detected every week.After 5 weeks,the ratio of bilateral kidney weight to body weight as well as a pathological section of kidney were observed.Results The weight of rats decreased,the ratio of kidney weight to body weight increased,serum creatinine and urea nitrogen all increased,kidney color changed significantly,and kidney damage was identified by hematoxylin-eosin(HE)staining and urate staining in group C compared with the normal group.Conclusions In conclusion,intragastric administration of 100 mg/kg adenine+1500 mg/kg oteracil potassium in the morning and evening was the best way to establish a rat model of chronic hyperuricemia renal damage.

关 键 词：慢性高尿酸血症 肾损害 大鼠 尿酸 肌酐 尿素氮 

分 类 号：Q95-33[生物学—动物学]