非人哺乳动物细胞系身份鉴定的研究进展  

Authentication of non-human mammalian cell lines

作　　者：朱亮[1] 屠珏[1] 王德军[1] ZHU Liang;TU Jue;WANG Dejun(Laboratory Animal Research Center/Institute of Comparative Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究所,杭州310053

出　　处：《中国实验动物学报》2021年第3期413-416,共4页Acta Laboratorium Animalis Scientia Sinica

摘　　要：非人哺乳动物细胞系在医学生物学研究中正起着越来越重要的作用。与人源细胞一样,非人哺乳动物细胞在使用中也面临着错用、污染和变异的问题。但与人源细胞相比,非人哺乳动物细胞受到的关注比较少,细胞身份鉴定技术发展较慢。目前对非人哺乳动物细胞系身份鉴定的研究主要集中在小鼠、中国仓鼠和非洲绿猴3个物种,方法以短串联重复序列(short tandem repeats,STR)标记检测为主,单核苷酸多态性(single nucleotide polymorphism,SNP)分析为辅。本文就非人哺乳动物细胞系身份鉴定技术在不同物种中的发展现状进行综述。Non-human mammalian cell lines play an increasingly important role in medical and biological research.Like human cells,non-human mammalian cells face the problems of misuse,pollution and variation.However,compared with human cells,the authentication of non-human mammalian cell lines has received less attention and has not yet been fully explored.To date,research into the authentication of non-human mammalian cell lines has mainly focused on cell lines from mice,Chinese hamsters,and African green monkeys.The authentication method mainly use short tandem repeat(STR)markers,supplemented by single nucleotide polymorphism(SNP)analysis.Here,we review the development of the authentication of non-human mammalian cell lines in different species.

关 键 词：非人细胞系 细胞系身份鉴定 质量控制 短串联重复序列 

分 类 号：Q95-33[生物学—动物学]