低温及光照对彩叶杨树叶片中花色素苷变化的影响  

Effects of low temperature and light involved in change of anthocyanin in leaves of color-leaved poplar

作　　者：朱秀红[1] 蔡曜琦 杨金橘 程红梅 茹广欣[1] ZHU Xiu-hong;CAI Yao-qi;YANG Jin-ju;CHENG Hong-mei;RU Guang-xin(College of Forestry,Henan Agricultural University,Zhengzhou 450000,China)

机构地区：[1]河南农业大学林学院,郑州450000

出　　处：《湖北农业科学》2021年第12期85-89,共5页Hubei Agricultural Sciences

基　　金：国家林草局生物安全与遗传资源项目(KJZXSA2019042);河南省林木种质资源建设项目(30601998)。

摘　　要：以5个彩叶杨树品种为试验材料,对花色素苷含量、叶绿素含量、类胡萝卜素含量、可溶性糖含量、蔗糖含量以及花色素苷相关酶活性进行测定,探究低温及光照是否参与彩叶杨树叶片中花色素苷的变化。结果表明,相对低温引起彩叶杨树叶片内叶绿素和类胡萝卜素含量呈下降趋势,花色素苷含量及糖含量均呈上升趋势。随着花色素苷含量的上升,叶片的抗氧化能力上升。花色素苷合成相关酶活性(PAL、CHI、DFR、UFGT)也呈不同程度的上升,引起花色素苷进一步合成积累;同时相对低温促进彩叶杨树花色素苷积累的反馈机制必须有光照参与,当进行低温完全遮光处理时,5个品种花色素苷含量下降至初始水平,蔗糖及可溶性糖含量大幅度下降,相关酶活性也下降至较低水平,重新通入光源后,均重新上升至较高水平。Using five color-leaved poplar varieties as experimental material,anthocyanin,chlorophyll,carotenoid content,soluble sugar content,sucrose content and anthocyanin related enzyme activity were determined to investigate whether low temperature and light were involved in change of anthocyanins in the leaves of color-leaved poplar.The results showed that the contents of chlorophyll and carotenoids in the leaves of color-leaved poplar showed a decreasing trend due to relatively low temperature,while the contents of anthocyanin and sugar content increased.With the increase of anthocyanin content,the antioxidant capacity of leaves increased.The activity of enzymes related to anthocyanin(PAL,CHI,DFR,UFGT)also increased to different degrees,causing further accumula⁃tion of anthocyanin.At the same time,the feedback mechanism of relative low temperature promoting anthocyanin accumulation in col⁃or-leaved poplars must involve light,the content of anthocyanin,sucrose and soluble sugar decreased greatly,and the activity of relat⁃ed enzymes also decreased to a lower level when completely shading was applied at low temperature.After re-feeding the light source,these indicators re-rose to a higher level.

关 键 词：彩叶杨树 花色素苷 低温 光照 酶活性 

分 类 号：S792.11[农业科学—林木遗传育种]