SPE-HPLC法测定雷公藤半成品及制剂中四种成分的含量  

Determination of four components in semi-finished products and preparations of Triperygium wilfordii by SPE-HPLC

作　　者：肖安菊 龚达林 刘三波 余浩 喻昕 XIAO An-ju;GONG Da-lin;LIU San-bo;YU Hao;YU Xin(College of Medicine,Hubei Polytechnic University,Huangshi 435003,Hubei,China;China Resources Sanjiu(Huangshi)Pharmaceutical Co.,Ltd.,Huangshi 435004,Hubei,China)

机构地区：[1]湖北理工学院医学院,湖北黄石435003 [2]华润三九(黄石)药业有限公司,湖北黄石435004

出　　处：《湖北农业科学》2021年第12期131-134,共4页Hubei Agricultural Sciences

基　　金：湖北省卫生健康委中医药科研项目(ZY2019M070)。

摘　　要：采用BRP固相萃取小柱纯化、高效液相色谱法同时检测雷公藤干浸膏、雷公藤片、雷公藤多苷片中四种成分的含量,采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,乙腈、甲醇、0.5%磷酸、水作为流动相梯度洗脱,建立固相萃取-高效液相色谱法同时测定雷公藤半成品、制剂中雷公藤甲素、雷公藤红素、去甲泽拉木醛和雷公藤内酯甲4种成分含量测定方法。结果表明,雷公藤甲素、雷公藤红素、去甲泽拉木醛和雷公藤内酯甲的线性关系良好(r≥0.996),加样回收率平均值分别为92.50%、94.93%、99.36%、92.29%,RSD分别为4.70%、6.41%、5.90%、4.36%。To establish a method for the simultaneous determination of triptolide,tripterine,demethylzeylasteral and wilforlide A in semi-finished products and preparations of Triperygium wilfordii by SPE-HPLC.The contents of four components in Triperygium wil⁃fordii extractum,Triperygium wilfordii tablets and Triperygium glycosides tablets were determined by HPLC,The separation was achieveds uning Kromasil C18(250 mm×4.6 mm,5μm)with an acetonitrile,methanol,0.5%phosphoric acid and water gradient elu⁃tion system.Results showed that the linear relationship of triptolide,tripterine,demethylzeylasteral and wilforlide A were good(r≥0.996).The average recoveries of the samples were 92.50%,94.93%,99.36%,92.29%,RSD were 4.70%,6.41%,5.90%,4.36%.

关 键 词：雷公藤干浸膏 雷公藤片 雷公藤多苷片 固相萃取 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]