重庆2017-2019年HIV抗体筛查有反应性样本确证检测结果分析  

Analysis of confirmation results of HIV antibody positive samples with primary screening in chongqing from 2017 to 2019

作　　者：张媛媛 黄为[1] 刘华 马小松 周全华[1] ZHANG Yuanyuan;HUANG Wei;LIU Hua;MA Xiaosong;ZHOU Quanhua(Chongqing Center for Disease Control and Prevention,Chongqing 400042,China;Chongqing Preventive Medicine Association,Chongqing 400042,China)

机构地区：[1]重庆市疾病预防控制中心,重庆400042 [2]重庆市预防医学会,重庆400042

出　　处：《现代医药卫生》2021年第13期2161-2165,共5页Journal of Modern Medicine & Health

基　　金：重庆市技术创新与应用发展专项面上项目(cstc2019jscx-msxmX0225)。

摘　　要：目的分析重庆市2017-2019年人类免疫缺陷病毒(HIV)抗体筛查有反应样本的确证结果,为重庆市艾滋病防治工作提供基础数据和科学依据。方法2020年6月收集重庆市艾滋病确证中心实验室2017-2019年HIV抗体筛查有反应性血液样本9010份,其中有反应性4754份,采用酶联免疫吸附试验和快速检测进行复检,任一复检试验有反应性的样本再采用免疫印迹法或重组免疫印迹法进行确证。结果有反应性4754份样本中无患者个人信息253份、既往感染者112份,故对4389份样本进行确证,其中确证阳性4227份,不确定87份,阴性75份。4227例样本中以男性为主(81.94%),年龄分布主要集中在20~<30、≥60岁,门诊和医院患者为主要来源(41.19%)。免疫印迹法检测结果中gp160阳性率最高(100.00%),重组免疫印迹法检测结果中gp41阳性率最高(100.00%)。追踪到55份不确定样本已转阳,其首次检测结果中含gp160、p24、gp41转阳率较高。结论重庆市艾滋病确证中心实验室2017-2019年主要送检人群为门诊和住院患者,确证结果阳性率较高,确证阳性条带分析膜蛋白基因带阳性率最高,应重视确证中不确定样本的随访及追踪。Objective To provide basic data and scientific evidence for HIV prevention and treatment in Chongqing,the confirmation data of HIV antibody primary screening positive samples from Chongqing confirmation central laboratory with period 2017-2019,were analyzed.Methods A total of 9010 responsive blood samples for HIV antibody screening from 2017 to 2019 were collected from the laboratory of Chongqing AIDS Conclusively Center in June 2020,among which 4754 were reactivity.ELISA and RT were performed for re-examining HIV antibody screening positive samples,then reinspection tests were performed by using WB or RIBA analysis after the reactivity samples were presented.Results Among the 4754 reactive samples,there were no personal information of 253 patients and 112 previous infected patients.Therefore,confirmation was conducted on 4389 samples,among which 4227 cases were confirmed positive,87 cases were uncertain,and 75 cases were negative.The 4227 samples were mainly male(81.94%),and their age distribution was mainly concentrated in 20-<30 years old and 60 years old and above.Samples originated mainly from outpatients and hospital patients(41.19%),the gp160 band appeared most frequently in WB-confirmed positive samples(100.00%).The gp41 band appeared most frequently in RIBA-confirmed positive samples(100.00%).Fifty-five uncertain samples had been traced that turned positive.Those with bands gp160,p24 and gp41 in the first test results had the higher positive conversion rate.Conclusion The samples of inspection crowd from the confirmation central laboratory of Chongqing are mostly to be outpatients and hospital patients from 2017 to 2019,the positive rate of HIV confirmatory test results is high.Analysis of confirmation positive strips,the positive rate of EVN is the highest.Attention should be paid to the follow-up of the uncertain samples in the confirmation tests.

关 键 词：HIV HIV抗体 临床实验室技术 免疫印迹法 确证实验 重庆 

分 类 号：R512.91[医药卫生—内科学]