一种马勃属野生真菌分离鉴定及生物学特性研究  

Study on Identification and Biological Characteristics of a Wild Lycoperdon perlatum

作　　者：张妍 ZHANG Yan(Liaoning Provincial Institute of Poplar,Gaizhou,Liaoning 115213)

机构地区：[1]辽宁省杨树研究所,辽宁盖州115213

出　　处：《安徽农业科学》2021年第13期120-123,共4页Journal of Anhui Agricultural Sciences

基　　金：辽宁省自然基金资助计划(2019-MS-205);辽宁省农业科学院基本科研业务费农业绿色高质量发展专项(2021HQ1912)。

摘　　要：采用形态鉴定与ITS分子序列鉴定相结合的方法,对采集的一株马勃属野生真菌进行鉴定,并对该菌种菌丝生物学特性进行研究。结果表明,初步通过形态鉴定法鉴定为梨形马勃,后进一步用ITS分子序列鉴定法鉴定,分离株rDNA ITS片段长度为697 bp,测序结果登录NCBI与GenBank中已知菌种进行BLAST比对,采用MEGA 2.0软件构建系统发育树,鉴定分离菌种为网纹马勃,结合形态特征,最终鉴定该菌种为网纹马勃(Lycoperdon perlatum)。生物学特性研究结果表明,可溶性淀粉为该菌丝生长的最适碳源,硝酸铵为最适氮源,7.0为最适pH,菌丝最适温度为25℃。A wild macrofungi fruiting body was isolated by tissue isolation method,identified by morphological identification and its molecular sequence identification,and the biological characteristics of the mycelium were studied.The results showed that the strain was identified as Lycoperdon pyriforme Schaeff.:Pers by morphological identification and ITS molecular sequence identification.The length of ITS fragment of rDNA was 697 bp.The sequencing results were registered in NCBI and compared with the known strains in GenBank by blast,the ITS sequence with similar height was downloaded and compared with cluster W.Mega 2.0 software was used to construct the system tree,and the isolated strain was identified as Lycoperdon perlatum.The results showed that the suitable carbon source for mycelium growth was soluble starch,the suitable nitrogen source was ammonium nitrate,the suitable pH value was 7.0,and the optimum temperature was 25℃.

关 键 词：网纹马勃 分离 鉴定 生物学特性 

分 类 号：S646[农业科学—蔬菜学]