基于线粒体DNA控制区序列的重庆岩原鲤人工养殖群体遗传多样性分析  

ANALYSIS ON GENETIC DIVERSITY OF CULTURED ROCK CARP(PROCYPRIS RABAUDI)FROM CHONGQING CITY BASED ON THE MTDNA D-LOOP SEQUENCES

作　　者：岳华梅[1] 阮瑞[1] 曹宏[2] 周莉[2] 蒋伟 李双 李创举[1] YUE Hua-Mei;RUAN Rui;CAO Hong;ZHOU Li;JIANG Wei;LI Shuang;LI Chuang-Ju(Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;Chongqing Fishery Sciences Research Institute,Chongqing 400020,China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业部淡水生物多样性保护重点实验室,武汉430223 [2]中国科学院水生生物研究所,武汉430072 [3]重庆市水产科学研究所,重庆400020

出　　处：《水生生物学报》2021年第4期846-850,共5页Acta Hydrobiologica Sinica

基　　金：重庆市农业投资集团有限公司院士工作站技术服务项目(2017)资助。

摘　　要：为研究重庆市长寿和涪陵地区岩原鲤(Procypris rabaudi)人工养殖群体的遗传多样性,采用PCR扩增获得175尾岩原鲤个体的线粒体DNA控制区(mtDNA D-loop)部分序列片段。对其中692 bp序列分析共发现11个单倍型,其中Hap1为主要单倍型,占总样本的76.57%。单倍型Hap10演化速率较快,并且与单倍型Hap6遗传距离最远。长寿及涪陵地区岩原鲤人工群体的单倍型多样性分别为0.4100±0.0550和0.3970±0.0820,核苷酸多样性分别为0.0013±0.0003和0.0013±0.0004。通过与长江上游江段野生群体(苍溪江段、合江江段、木洞江段、通江江段、万州江段、武隆江段、习水河和唐河)及北碚区人工养殖群体的遗传多样性进行比较,发现岩原鲤长寿及涪陵地区人工养殖群体遗传多样性明显低于野生群体,但略高于北碚人工养殖群体。为保持岩原鲤人工养殖群体的遗传多样性,应尽量选择遗传距离较远的亲本进行配对,并定期补充不同遗传背景的个体作为后备亲鱼。To assess the genetic diversity of cultured rock carp Procypris rabaudi from Changshou and Fulin districts in Chongqing city,partial DNA sequences of mtDNA control region(D-loop)were amplified from 175 individuals by PCR.A total of eleven haplotypes were determined by sequence alignments of the amplified 692 bp sequences.Among them,Hap1 was the main haplotype,accounting for 76.57%of the entire individuals.The haplotype of Hap10 possessed the highest evolutionary rate,and shared the farthest evolutionary distance with Hap6.The haplotype diversities of cultured P.rabaudi from Changshou and Fulin districts were 0.4100±0.0550 and 0.3970±0.0820,respectively,and their nucleotide diversities were 0.0013±0.0003 and 0.0013±0.0004,respectively.The genetic diversities were compared with wild populations from the upper Yangtze River drainage(including reaches of Cangxi,Hejiang,Wanzhou,Wulong,Xishui and Tanghe),and cultured population from Beibei district.The results indicated that the genetic diversity of cultured P.rabaudi from Changshou and Fulin districts was obviously lower than that of wild populations,but slightly higher than that of cultured population from Beibei district.To sustain the genetic diversity of cultured rock carp,it is necessary to choose parent fish with further genetic distances for propagation,and to supplement new broodstocks with different genetic background as well.

关 键 词：线粒体DNA控制区 遗传多样性 单倍型 人工养殖群体 岩原鲤 

分 类 号：Q346.5[生物学—遗传学]