桂东南地区三种常见鸡病毒性免疫抑制病的血清抗体检测与分析  

Detection and Analysis of Serum Antibody Against Three Kinds of Common Viral Immunosuppressive Diseases in Chicken Flocks in the Region of Southeast Guangxi

作　　者：吕其壮 李佩伦 张成成 覃婷 陈旭健 LYU Qizhuang;LI Peilun;ZHANG Chengcheng;QIN Ting;CHEN Xujian(College of Biology&Pharmacy,Yulin Normal University,Yulin,Guangxi 537000,China;Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Yulin,Guangxi 537000,China;Animal Diseases Control&Prevention Centre of Yulin City,Yulin 537000,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]玉林师范学院生物与制药学院,广西玉林537000 [2]广西农产资源化学与生物技术重点实验室,广西玉林537000 [3]玉林市动物疫病预防控制中心,广西玉林537000 [4]扬州大学兽医学院,江苏扬州225009

出　　处：《福建农业学报》2021年第5期506-511,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31860708);广西自然科学基金项目(2017GXNSFBA198025);玉林师范学院大学生创新创业训练计划项目(202010606226)。

摘　　要：【目的】了解3种常见病毒性免疫抑制病在桂东南地区鸡群中的发病情况和流行趋势。【方法】采用酶联免疫吸附试验(ELISA)方法,对2018年3月至2019年4月来自玉林、北流、兴业、博白、容县、陆川、贵港、桂平、平南等9个县市农村散养户送检的637份病鸡血清样品进行病毒抗体的检测并分析地区、季节、品种和年龄段等因素对鸡群感染病毒性免疫抑制病的影响。【结果】以上地区的农户散养鸡群中存在3种常见病毒性免疫抑制病的抗体,且不同病毒的抗体情况受地区、季节、品种和年龄段等因素的影响。鸡传染性贫血病毒(CIAV)、禽网状内皮组织增生症病毒(REV)和禽呼肠孤病毒(ARV)的抗体总阳性率分别为23.86%、13.97%、7.06%,提示CIAV在以上地区鸡群中的感染率相对较高,应该作为今后该地区鸡群疫病防控的重点。此外,在所有阳性样品中混合感染率为24.65%,说明目前以上地区农户散养鸡群中的病毒性免疫抑制病存在混合感染,尤其以CIAV与其他病原组成的混合感染类型为主,占98.11%。【结论】针对桂东南地区农户散养鸡群进行血清学调查,为防控该地区鸡群中的3种常见病毒性免疫抑制病提供了重要参考。【Objective】In order to explore the current morbidity and epidemic trend of three kinds of common viral immunosuppressive diseases in chicken flocks in southeast Guangxi.【Method】From March 2018 to April 2019,a total of 637 sick chicken sera were collected from scattering chicken raising households in nine counties and cities including Yulin,Beiliu,Xingye,Bobai,Rongxian,Luchuan,Guigang,Guiping and Pingnan,to detect the serum antibodies against three viruses by an enzyme-linked immunosorbent assay(ELISA)and analyzed the impact of different factors including region,season,breed and age on the infection statuses of these three viral immunosuppressive diseases.【Result】The results showed that the antibodies against three common immunosuppressive viruses were ubiquitous in the above areas and the positive rates of antibodies against different viruses were affected by regions,seasons,breeds and age groups of the chicken flocks.The positive rates of antibodies against Chicken infectious anemia virus(CIAV),Reticuloendotheliosis virus(REV)and Avian reovirus(ARV)were 23.86%,13.97%and 7.06%respectively,indicating that the positive rate of CIAV were higher than other two viral immunosuppressive diseases in chicken flocks of the farmers in the above-mentioned regions and it should be regarded as the focus of the prevention and control of epidemic diseases in chicken flocks.In addition,multi-infection was the common infection type,as the mixed infection rate reached up to 24.65%,and CIAV co-infection with other pathogens were the most common diversified infection type,accounting for 98.11%.【Conclusion】This study provided the serological surveillance of chicken viral immunosuppressive diseases in the chicken flocks of the scattering raising households in the region of southeast Guangxi and provided a reliable reference for making control measures of these three kinds of common viral immunosuppressive diseases.

关 键 词：鸡 病毒性免疫抑制病 抗体检测 ELISA 

分 类 号：S851.31[农业科学—预防兽医学] S858.31[农业科学—兽医学]