芒果葡萄座腔菌对3种杀菌剂的敏感性测定  

Susceptibility of Botryosphaeriaceous isolates from mango to three fungicides

作　　者：赵江 余知和[1] 李其利[2,3] 唐利华 郭堂勋[2,3] 黄穗萍 莫贱友[2,3] 孙明艳 ZHAO Jiang;YU Zhi-he;LI Qi-li;TANG Li-hua;GUO Tang-xun;HUANG Sui-ping;MO Jian-you;SUN Ming-yan(College of Life Sciences,Yangtze University,Jingzhou,Hubei 434023,China;Institute of Plant Protection,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China;Flowers Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434023 [2]广西农业科学院植物保护研究所,南宁530007 [3]广西作物病虫害生物学重点实验室,南宁530007 [4]广西农业科学院花卉研究所,南宁530007

出　　处：《南方农业学报》2021年第4期984-994,共11页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31600029,31560526);广西自然科学基金项目(2016GXNSFCB38000)。

摘　　要：【目的】明确从我国芒果主产区分离获得的89株葡萄座腔菌对3种杀菌剂的敏感性,以期为葡萄座腔菌引起的芒果病害防控提供参考。【方法】采用菌丝生长速率法对采自广西、海南、云南、四川、广东和福建芒果主产区的89株芒果葡萄座腔菌进行咪鲜胺、苯醚甲环唑和吡唑醚菌酯的敏感性测定,并利用DPS 9.01和SPSS 20分析供试菌株对3种药剂的敏感性及交互抗性。【结果】供试89株芒果葡萄座腔菌对咪鲜胺、苯醚甲环唑和吡唑醚菌酯的敏感性存在差异,其中对咪鲜胺的EC_(50)介于0.0048~38.5037 mg/L,平均值为2.8637 mg/L,最大EC_(50)是最小EC_(50)的8021.6倍;对苯醚甲环唑的EC_(50)介于0.0147~8.8935 mg/L,平均值为1.1761 mg/L,最大EC_(50)是最小EC_(50)的605.0倍;对吡唑醚菌酯的EC_(50)介于0.0195~145.0578 mg/L,平均值为8.1939 mg/L,最大EC_(50)是最小EC_(50)的7438.9倍。采自不同芒果产区的芒果葡萄座腔菌株对咪鲜胺、苯醚甲环唑和吡唑醚菌酯的敏感性存在差异,其中采自广东的菌株对咪鲜胺和吡唑醚菌酯的平均EC_(50)最大,分别为8.1127和15.7240 mg/L,采自四川的菌株对苯醚甲环唑的平均EC_(50)最大,为1.6730 mg/L。不同属的芒果葡萄座腔菌菌株对咪鲜胺、苯醚甲环唑和吡唑醚菌酯的敏感性存在差异,其中毛色二孢属(Lasiodiplodia)菌株对3种杀菌剂的平均EC_(50)均高于葡萄座腔菌属(Botryosphaeria)和新壳梭孢属(Neofusicoccum)菌株的平均EC_(50)。【结论】采自我国不同芒果产区的89株芒果葡萄座腔菌菌株对苯醚甲环唑较敏感,咪鲜胺次之,对吡唑醚菌酯的敏感性最低。供试芒果葡萄座腔菌对3种杀菌剂无交互抗性,可合理混合或交替施用。不同来源、不同属的芒果葡萄座腔菌对3种杀菌剂的敏感性存在差异,生产中应根据具体的区域及病原菌种类合理地科学施用。【Objective】To determine the susceptibility of 89 strains of Botryosphaeriaceous isolate from major mango producing areas in China to three fungicides:prochloraz,difenoconazole and pyraclostrobin,and to provide reference for mango disease control caused by Botryosphaeriaceous isolate.【Method】Mycelial growth rate method was used to determine the sensitivity of Botryosphaeriaceous isolate collected from main mango producing areas of Guangxi,Hainan,Yunnan,Sichuan,Guangdong and Fujian to prochloraz,difenoconazole and pyraclostrobin,and DPS9.01 and SPSS20 softwares were used to analyze the sensitivity and cross resistance of the strains to the three fungicides.【Result】Among the 89 strains tested in this study,there were differences in susceptibility to prochloraz,difenoconazole and pyraclostrobin.The EC_(50) for prochloraz ranged from 0.0048 to 38.5037 mg/L,with an average value of 2.8637 mg/L,and the maximum EC_(50) was 8021.6 times of the minimum EC_(50).The EC_(50) for difenoconazole ranged from 0.0147 to 8.8935 mg/L,with an average value of 1.1761 mg/L,and the maximum EC_(50) was 605.0 times of the minimum EC_(50).The EC_(50) values for pyraclostrobin ranged from 0.0195 to 145.0578 mg/L,with an average value of 8.1939 mg/L,and the maximum EC_(50) was7438.9 times of the minimum EC_(50).There were differences in the susceptibility of the isolates collected from different mango producing areas to prochloraz,difenoconazole and pyraclostrobin,among which the isolates from Guangdong had the highest average EC_(50) to prochloraz(8.1127 mg/L)and pyraclostrobin(15.7240 mg/L),and the isolates from Sichuan had the highest average EC_(50) to difenoconazole(1.6730 mg/L).There were differences in the susceptibility of Botryosphaeriaceous isolates from different genera to prochloraz,difenoconazole and pyraclostrobin.The average EC_(50) of the isolates in Lasiodiplodia to three fungicides was higher than that of Botryosphaeria and Neofusicoccum.【Conclusion】All the 89 strains of Botryosphaeriaceous isolates collecte

关 键 词：葡萄座腔菌 葡萄座腔菌属 毛色二孢属 新壳梭孢属 咪鲜胺 苯醚甲环唑 吡唑醚菌酯 敏感性 

分 类 号：S436.679[农业科学—农业昆虫与害虫防治]