阿胶对慢性阻塞性肺疾病大鼠肺功能及肺组织病理损伤的影响  

Effects of Colla corii asini on lung function and pathological injury in rats with chronic obstructive pulmonary disease

作　　者：那扎开提·艾尼瓦尔 胡广 张田甜[1] 宋美月 赵红梅 靳洪涛[3] 王婧[1] Nazhakaiti·AINIWAER;HU Guang;ZHANG Tian-tian;SONG Mei-yue;ZHAO Hong-mei;JIN Hong-tao;WANG Jing(Department of Pathophysiology,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005;Beijing University of Chinese Medicine,Beijing 100029;New Drug Safety Evaluation Center,Institute of Materia Medica,CAMS&PUMC,Beijing 100050,China)

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院病理生理学系,北京100005 [2]北京中医药大学,北京100029 [3]中国医学科学院北京协和医学院药物研究所新药安全评价研究中心,北京100050

出　　处：《基础医学与临床》2021年第7期970-974,共5页Basic and Clinical Medicine

基　　金：国家重点研发计划(2018YFC1315103);国家自然科学基金(82074104)。

摘　　要：目的探究阿胶(CCA)对慢性阻塞性肺疾病(COPD)大鼠肺功能及肺组织病理损伤的影响。方法将25只大鼠随机分成对照组、模型组、阿胶(低、中、高)剂量组(1、2、4 g/kg),每组5只。采用卷烟烟雾暴露法造模,烟雾浓度为(1100±10)mg/m3,每日1次,每次90 min,连续48周。造模结束次日起,每日灌胃治疗。连续治疗28 d后,用动物肺功能仪检测肺功能,HE染色观察肺组织病理。结果与对照组相比,模型组大鼠潮气量(TV)、每分钟呼气量(EV)、呼气持续时间(Te)、松弛时间(RT)、呼气末期停顿(EEP)和支气管收缩程度(Penh)显著增加(P<0.05),而呼气峰流速(PEF)、吸气峰流速(PIF)、每分钟通气量(MV)、呼出50%潮气量时呼气流速(EF50)和呼吸频率[f(B)]显著降低(P<0.05);模型组大鼠出现肺气肿和炎性病理性改变;肺组织病变加重。与模型组相比,阿胶高剂量组Te、RT、Penh显著下降(P<0.05),MV、PIF、EF50显著增加(P<0.05);肺部炎性细胞浸润减少;肺泡扩张减轻;肺大泡减少;肺气肿程度减轻;肺组织病理损伤明显缓解。结论CCA可以改善COPD大鼠肺功能、减轻肺组织炎性反应。Objective To explore the effects of Colla corii asini(CCA)(common name E’jiao)on lung function and pathological injury of the lung tissue in rats with chronic obstructive pulmonary disease(COPD).MethodsTwenty-five rats were randomly divided into the control group,COPD model group and treatment group with low,medium and high-doses of CCA(1,2,4 g/kg),with 5 rats in each group.The COPD model group and treatment groups were modeled by cigarette smoke exposure with a concentration of(1100±10)mg/m3 once a day,90 minutes each time,for 48 consecutive weeks.After the end of the next day,the CCA groups were given by daily oral gavage treatment for 28 days.Lung function was detected by animal lung function instrument and the pathological changes of the lung tissue were assessed by HE staining and microscopy.Results Compared with the control group,TV,EV,Te,RT,EEP and Penh were significantly increased in the COPD model group(P<0.05),while PEF,PIF,MV,EF50 and f(B)were significantly decreased(P<0.05).Inflammation and pathological changes of emphysema wereobserved,and lung tissue lesions were aggravated.Compared with the COPD model group,Te,RT and Penh were significantly reduced in the high-dose CCA group(P<0.05),while MV,PIF and EF50 were significantly higher(P<0.05).Less infiltration of inflammatory cells,less alveolar expansion and fewer pulmonary bullae were observed in high-dose CCA-treated rats.The degree of emphysema was reduced,and pathological damage to lung tissue was clearly alleviated.ConclusionsCCA improves lung function and alleviates the inflammatory response of the lung tissue in COPD rats.

关 键 词：阿胶 慢性阻塞性肺疾病 肺功能 肺损伤 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统]