外源海藻糖对NaHCO_(3)胁迫下甘草幼苗生长调节及总黄酮含量的影响  

Effects of exogenous trehalose on growth regulation and total flavonoid content of Glycyrrhiza uralensis seedlings under NaHCO_(3) stress

作　　者：柳福智[1] 张迎芳 陈垣[1] LIU Fu-zhi;ZHANG Ying-fang;CHEN Yuan(College of Agriculture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070

出　　处：《草业学报》2021年第7期148-156,共9页Acta Prataculturae Sinica

基　　金：道地药材安全生产及绿色加工(2016C-15);甘肃省现代农业中药材产业体系首席专家(GARS-ZYC-1)资助。

摘　　要：本研究以甘草无菌苗为试验材料,采用植物组织培养的方法,分析50 mmol·L^(-1) NaHCO_(3)胁迫下外源海藻糖对甘草幼苗生长量、叶绿素含量、渗透调节物质含量、抗氧化保护酶活性和总黄酮含量的影响。结果表明:50 mmol·L^(-1) NaHCO_(3)胁迫显著降低了甘草幼苗的生长量、叶绿体色素含量、K^(+)浓度、抗氧化保护酶(SOD、POD、CAT)活性和总黄酮含量,显著提高了丙二醛、脯氨酸和可溶性糖含量以及Na^(+)浓度;施加15 mmol·L^(-1)海藻糖可显著提高甘草幼苗的生长量,提高叶绿素含量、K^(+)浓度和总黄酮含量,降低丙二酸含量、脯氨酸含量、可溶性糖含量和Na^(+)浓度,并且提高抗氧化保护酶活性。因此,NaHCO_(3)胁迫下施加外源海藻糖对甘草幼苗生长具有良好的调节作用,可以增强甘草的抗碱能力,促进甘草幼苗生长。本研究为外源海藻糖提高甘草耐碱性和揭示其调控机制提供理论依据。In this study,aseptic plantlets of Glycyrrhiza uralensis generated by tissue culture were used to study the stress effects of NaHCO_(3) at 50 mmol·L^(-1) with and without exogenous trehalose addition to mitigate stress effects.The biomass,chlorophyll content,concentrations of osmotic regulation substances,antioxidant enzyme activities and total flavonoid contents in the plantlets were monitored.It was found that NaHCO_(3)-stressed seedlings showed significantly lower biomass,chlorophyll content,concentration of K^(+),total flavonoid contents and antioxidant enzyme(superoxide dismutase,peroxidase,catalase)activities,and significantly higher malondialdehyde(MDA),proline,soluble sugar and Na^(+)concentrations.Those plantlets treated with 15 mmol·L^(-1)trehalose showed significantly improved biomass,chlorophyll content,concentration of K^(+),total flavonoid content and antioxidant enzyme activities,together with reduced MDA,proline,soluble sugar,and Na^(+)concentrations.In summary,the application of exogenous trehalose under NaHCO_(3) stress had a major beneficial effect on the growth of G.uralensis plantlets and enhanced their alkali-resistance ability of the seedlings.This study provides a theoretical basis for understanding the mechanism of exogenous trehalose enhancement of the alkali-resistance capacity of G.uralensis plantlets.

关 键 词：外源海藻糖 NAHCO3胁迫 甘草幼苗 渗透调节物质 抗氧化保护酶 

分 类 号：S567.71[农业科学—中草药栽培]