CRISPR/Cas9基因编辑技术在病毒感染性疾病研究中的应用  

The application of CRISPR/Cas9 gene editing technology in the study of viral infective diseases

作　　者：雷恩 苗明三[1] 曹艺明 杨静[1,3] LEI En;MIAO Ming-san;CAO Yi-ming;YANG Jing(Henan University of Chinese Medicine,Zhengzhou 450046,China;Shandong University of Chinese Medicine,Jinan 250355,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]河南中医药大学,郑州450046 [2]山东中医药大学,济南250355 [3]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《军事医学》2021年第5期384-389,共6页Military Medical Sciences

基　　金：国家自然科学基金(81830101,81703429)。

摘　　要：自然环境中的易感病毒通过呼吸道、消化道、皮肤等侵入机体,进而在宿主细胞大量增殖,造成机体不同程度的损伤,严重时危及生命。目前大多数病毒感染性疾病均较难根治,也无特效药,已成为世界各国研究者面临的挑战。CRISPR/Cas9基因编辑技术通过gRNA特异性识别病毒基因组或宿主靶基因位点,促使Cas9核酸酶精准切割互补双链DNA,从而达到编辑目的基因的目的。该文主要综述了CRISPR/Cas9系统的技术原理及其在病毒感染性疾病研究中的应用。The susceptible viruses in the natural environment invade the body through the respiratory tract,digestive tract,skin and so on before they proliferate in large numbers in host cells,causing different degrees of damage to the body and posing a threat to human lives,in serious cases.Most viral infective diseases are difficult to cure,and there is no specific drug available,which has become a challenge faced by researchers globally.CRISPR/Cas9 gene editing technology can specifically identify viral genome or host target gene sites through gRNA,and promote Cas9 nuclease to precisely cut complementary double-stranded DNA so as to serve the purpose of editing target genes.In this paper,the technical principles of CRISPR/Cas9 system and its application in the study of viral infective diseases are reviewed.

关 键 词：CRISPR/Cas9 基因编辑 病毒感染性疾病 基因组 冠状病毒感染 

分 类 号：K373[历史地理—历史学]