基于光学相干层析技术的昆虫胚胎影像处理方法  

Image Processing Method of an Insect Embryo Based on Optical Coherence Tomography

作　　者：买童童 魏丽亚 姚晓天 苏亚 王秀丽[1,2] 杨丽君[1] Mai Tongtong;Wei Liya;X.Steve Yao;Su Ya;Wang Xiuli;Yang Lijun(College of Physics Science&Technology,Hebei University,Baoding,Hebei 071002,China;Hebei Provincial Center for Optical Sensing Innovations,Baoding,Hebei 071002,China;Institute of Life Science and Green Development,Baoding,Hebei 071002,China;College of Life Sciences,Hebei University,Baoding,Hebei 071002,China)

机构地区：[1]河北大学物理科学与技术学院,河北保定071002 [2]河北省光学感知技术创新中心,河北保定071002 [3]河北大学生命科学与绿色发展研究院,河北保定071002 [4]河北大学生命科学学院,河北保定071002

出　　处：《中国激光》2021年第9期179-186,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(31702064);河北省重点研发计划(19212109D,20542201D);河北省自然科学基金(F2020201041,C2018201135);河北省高等学校科学技术研究基金(QN2017022,QN2019035);河北省教育厅青年基金(QN2019117);河北大学自然科学多学科交叉研究计划资助项目(DXK201910);河北大学创新训练项目(2020043);河北大学“新医科”教学改革项目(2020XYKY08)。

摘　　要：胚胎在发育过程中会逐渐形成具有不同组织和器官的复杂生物体。对胚胎发育的无创实时监测有助于深入了解其形态演变过程和发育规律。光学相干层析(OCT)技术具有无损、高分辨、三维成像等特点,非常适合用于对生物体胚胎的发育情况进行监测。然而,当采用OCT技术检测昆虫胚胎时,虫卵外壳的存在会影响OCT三维重建图的可视化效果,使得卵内部胚胎的三维图无法清晰地呈现。基于此,本文结合OCT成像特点提出了一种消除卵壳影响的图像处理方法。该方法可以自动识别出卵壳区域并予以“擦除”,从而只呈现昆虫卵内部胚胎的清晰三维图像。该图像处理方法对于采用OCT技术研究昆虫胚胎发育具有重要的参考价值。Objective Embryonic development has attracted increasing attention in biological research.Monitoring the internal organs and tissues of an embryo is relevant to the study of embryonic development.Generally,two methods are employed to monitor tissues in the embryo:tissue section technology and stereomicroscope observation.Both methods need to kill the embryo and cannot monitor a single living embryo continuously.Therefore,noninvasive real-time monitoring of embryonic development is expected to understand the morphological evolution process.Optical coherence tomography(OCT)is a relatively novel imaging method in the 21st century and is based on the principle of low coherence interference.It uses near-infrared light to scan the sample noninvasively,and then a three-dimensional image reconstruction is performed.OCT can provide internal structure information with a depth of 1--12 mm below the sample surface.Several research groups have reported studies on the embryonic development of different animals,such as Xenopus,mice,and birds,using OCT.However,there are few reports on the application of OCT to insect embryonic development.The main reason is that insect embryos are usually wrapped with eggshells that protect the embryo from external damage.This morphology of the insect embryo significantly affects the real-time monitoring of its development.Therefore,a method that can automatically identify the edge and thickness of the eggshell and intelligently erase it is required.Methods An image processing method is proposed in this paper to eliminate the effect of eggshells on OCT imaging.This method can automatically identify and erase the area of the eggshells so that the clear three-dimensional image of the embryo inside the eggshell is presented.As shown in Fig.1,first,the three-dimensional image is filtered to reduce noise;second,local threshold segmentation and boundary recognition are performed;and then the position and thickness of eggshells can be extracted and erased in the original image.Afterward,the three-dimen

关 键 词：生物光学 光学相干层析成像 昆虫胚胎可视化 图像处理 

分 类 号：TN247[电子电信—物理电子学]