慢性乙型肝炎患者HBV-DNA载量与血清肝纤维化指标的关系  

作　　者：徐锋[1] 程德勇[1] 蒋贻乾 肖强古 

机构地区：[1]浙江省杭州市萧山区第一人民医院,311220

出　　处：《浙江临床医学》2021年第6期888-889,共2页Zhejiang Clinical Medical Journal

摘　　要：目的探讨慢性乙型肝炎悲者乙肝病毒脱氧核糖核酸(HBV-DNA)含量与血清肝纤维化指标的相关性。方法纳入84例慢性乙型肝炎患者作为研究对象,采用荧光定量聚合酶链式反应(PCR)法检测其HBV-DNA含量,采用放射免疫法检测患者透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCII)及Ⅳ型胶原(CIV)等肝纤维化指标的水平。根据测得的HBV-DNA载量将所有患者分为A组(>10^(5)IU/ml)、B组(≥10^(3)IU/ml且≤10^(5)IU/ml)、C组(<10^(3)IU/ml),比较三组的临床资料与血清纤维化指标水平,分析HBV-DNA载量与血清纤维化指标的关系。结果三组患者的性别、年龄、体重指数、病程及肝功能分级等临床资料比较,差异均无统计学意义(P>0.05)。三组血清HA、LN、PCⅢ水平两两比较,差异均有统计学意义(P<0.05);A.B两组的CIN水平显著高于C组,差异有统计学意义(P<0.05)。HBV-DNA水平与HA(r=0.317,P=0.000)、LN(r=0.286,P=0.007)及PCM(r=0.348,P=0.000)呈显著相关,与CIV无明显相关(r=0.103,P-0.224)。结论慢性乙型肝炎患者HBV-DNA载量与血清肝纤维化指标具有一定相关性,监测其HBV-DNA水平与免疫功能可指导临床。Objective To explore the correlation between the content of hepatitis B virus deoxyibonucleic acid(HBV-DNA)and serum liver fibrosis indicators in patients with chronic hepatitis B.Methods Include 84 patents with chronic hepatitis B as the research object.Flurescence quantadive polymerase chain reaction method was used to dect HBV-DNA content,and radioimmumnoasay was used to detect hyaluronic acid and laminin of patients.The leved of liver fibrosis indicators such as type Ⅲ prollagen and type Ⅳ cllagen.According to the mcasured HBV-DNA load,all patients were divided into groupA(>10^(5)IU/ml),groupB(≥10^(3)IU/ml and≤10^(3)IU/ml),and groupC(<10^(5)IU/ml).The climical data and serum fibrosis index levels of the three groups were compared,and the relationship between HBV-DNA loud and serum fibrosis index was analyzed.Results There were no satistically significant differences in the clinical data of the three groups of patients,such as gender,age,body mass index,course of disease,and liver function cascationo(P>0.05).Serum HA.LN,and PCI levels of the three groups were compared in pais,and the diferencs were stitically signifcant(P<0.05);the CIV levels of the A and B groups were sigmific antly higher than that of the C group.and the dfferences were staitically signifcant(P<0.05).HBV-DNA level was sgnificantly orrelated with HA(r=0.317,P=0.000).LN(r=0.286,P=0.007)and PCIl(r=0.348,P=0.000),but not significantly correlated with CIN(r=0.103,P=0.224)Conclusion There is a certain correlation between HBV DNA level and serum liver fibrosis index in patients with chronic hepaitis B.It is helpful to evaluate the degree of liver fibrosis by monitoring HBV DNA level.

关 键 词：慢性乙型肝炎 乙肝病毒脱氧核糖核酸 血清纤维化指标 相关性 

分 类 号：R51[医药卫生—内科学]