浙江甘薯蔓枯病病原菌鉴定  

Identification of pathogen Diaporthe spp. associated with gummy stem blight of Ipomoea batatas in Zhejiang

作　　者：谢昀烨[1] 王会福[2] 应俊杰 方丽[1] 王连平[1] 王汉荣[1] XIE Yun-ye;WANG Hui-fu;YING Jun-jie;FANG Li;WANG Lian-ping;WANG Han-rong(Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Taizhou Academy of Agricultural Sciences,Linhai 317000,China;Xianju County Plant Protection and Quarantine Station,Xianju 317300,China)

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,杭州310021 [2]台州市农科院,临海317000 [3]仙居县植物保护检疫站,仙居317300

出　　处：《植物病理学报》2021年第3期441-445,共5页Acta Phytopathologica Sinica

基　　金：浙江省农业科学院地方科技合作项目(TZ2018009)。

摘　　要：甘薯(Ipomoea batatas(L.)Lam.)是我国重要的粮食作物,而且还是重要的休闲保健食品,同时也是浙江省主要的旱粮作物和经济作物[1]。近年来,在浙江省台州宁溪的甘薯上出现了一种毁灭性甘薯藤蔓枯死的"蔓枯"病害,该甘薯病害主要危害甘薯藤蔓,有时也危害叶片和薯块。Recent years,gummy stem blight caused by Diaporthe spp. is an important disease on Ipomoea batatas. In this study,infected plant tissues,collected from Ningxi,Taizhou,were used for isolating pathogens from the gummy stem. Three pure strains with similar cultural characteristics were isolated,tested for their pathogenicity against stems of I. batatas,and re-isolated from the tissue inoculated,and Koch’s postulate was fulfilled. The rRNA intergenic spacer(ITS)region of these isolates was amplified and the sequences were used for the phylogenetic analysis. The aliment result showed that the sequences(MT107556,MT107557 and MT107558)of three strains were 100% similarity with that of D. batatas CBS 122.21. These isolates were identified as D. batatas based on the morphological and molecular characteristics. Therefore,gummy stem blight on Ipomoea batatas was caused by D. batatas in Zhejiang province. Meanwhile,this study firstly showed that the stroma of D. batatas was coralloid.

关 键 词：旱粮作物 蔓枯病 病原菌鉴定 藤蔓 甘薯病害 经济作物 

分 类 号：S432.1[农业科学—植物病理学]