建兰花叶病毒TGBp1自身互作研究  

Studying homologous interactions of TGBp1 of cymbidium mosaic virus

作　　者：宋若楠 饶雪琴[1] SONG Ruo-nan;RAO Xue-qin(Guangdong Province Key Laboratory of Microbial Signals and Disease Control,College of Plant Protection,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省微生物信号与作物病害防控重点实验室,华南农业大学植物保护学院,广州510642

出　　处：《植物病理学报》2021年第3期478-482,共5页Acta Phytopathologica Sinica

摘　　要：建兰花叶病毒(cymbidium mosaic virus,CymMV)是危害兰花生产的重要病毒之一。CymMV属于马铃薯X病毒属(Potexvirus),基因组为正单链RNA,包含5个开放阅读框(open reading frames,ORFs);ORF 1编码依赖RNA的RNA聚合酶(RNA-dependent RNA polymerase,RdRp)。Cymbidium mosaic virus(CymMV)is one of the most important viruses that cause orchids great losses.Triple gene block protein 1(TGBp1)is a multifunctional protein of CymMV.Homologous interaction of CymMV TGBp1 is essential for its biological functions,but little is known about the site of homologous interaction of TGBp1.In this study,three truncated mutants of TGBp1-1(motif I,Ia),TGBp1-2(motif II,III,IV)and TGBp1-3(motif V,VI)were constructed based on its motifs respectively.Yeast two-hybrid(Y2 H),bimolecular fluorescence complementation(BiFC)and GST pull-down were used to screen the site of homologous interaction among three truncated mutants of TGBp1 and TGBp1.The results showed that TGBp1-2(motif II,III,IV)was the main site of homologous interaction of TGBp1.Our results provide a foundation for further research on the movement and replication of CymMV.

关 键 词：建兰花叶病毒 马铃薯X病毒 依赖RNA的RNA聚合酶 

分 类 号：S432.41[农业科学—植物病理学]