亚抑菌浓度亚胺培南对多重耐药鲍曼不动杆菌生物膜作用的研究  

Study on the Effect of Imipenem at Subinhibitory Concentration for the Biofilm of Multi-drug Resistant Acinetobacter Baumannii

作　　者：方甜甜[1] 宋沧桑[1] 刘德华[2] 唐树荣[2] 徐磊 李兴德[1] 杨焕芝[1] 丁逸欣 任宝军[2] FANG Tian-tian;SONG Cang-sang;LIU De-hua;TANG Shu-rong;XU Lei;LI Xing-de;YANG Huan-zhi;DING Yi-xin;REN Bao-jun(Department of Pharmacy,Kuming Municipal First People′s Hospital,Yunnan Kunming 650031,China;Clinical Laboratory Kunming First People′s Hospital,Yunnan Kunming 650031,China;Dali University,Yunnan Dali 671000,China;Kuming Medical University,Yunnan Kunming 650500,China)

机构地区：[1]昆明市第一人民医院药学部,云南昆明650031 [2]昆明市第一人民医院检验科,云南昆明650031 [3]大理大学,云南大理671000 [4]昆明医科大学,云南昆明650500

出　　处：《中国药物评价》2021年第3期224-228,共5页Chinese Journal of Drug Evaluation

基　　金：昆明市科技局项目(No.2019KJJH073);云南省教育厅科学研究基金项目(No.2019J1295);昆明市卫生科技人才培养项目[项2020-SW(后备)-36]。

摘　　要：目的:探讨亚抑菌浓度亚胺培南对多重耐药鲍曼不动杆菌生物膜的影响。方法:采用微量肉汤稀释法,测定医院2019年2月至2020年9月临床分离的36株亚胺培南耐药多重耐药鲍曼不动杆菌(MDR-AB)的最低抑菌浓度(MIC),结晶紫半定量染色法测定菌株生物膜形成水平。采用96孔聚苯乙烯板体外构建鲍曼不动杆菌生物膜的模型,用亚抑菌浓度亚胺培南作用于成熟生物膜。实验组Ⅰ、Ⅱ、Ⅲ组中分别用MIC、1/2MIC、1/4MIC的亚胺培南作用于已形成的成熟生物膜;对照组Ⅳ组不加亚胺培南。通过结晶紫半定量染色法对生物膜进行染色,共聚焦倒置显微镜下观察生物膜染色情况,采用多功能酶标仪在570 nm波长处测得生物膜OD值。结果:亚胺培南在实验组Ⅰ、Ⅱ、Ⅲ组中,即MIC、1/2MIC、1/4MIC浓度下作用于已成熟生物膜48、96 h后,所测得OD值与对照组Ⅳ组OD值之间进行比较,有显著性差异(P<0.05)。结论:亚抑菌浓度亚胺培南具有破坏成熟生物膜的能力,且随着浓度的升高,其破坏能力更强。Objective:To investigate the influnce of Imipenem in Sub-inhibitory concentration against the biofilm formation of Multiple drug resistant Acinetobacter Bauman(MDR-Ab).Methods:The minimum inhibitory concentration(MIC)of imipenem against 36 imipenem-resistant multidrug-resistant Acinetobacter baumannii(MDR-Ab)strains isolated from a third-class A hospital from February 2019 to September 2020 was determined by microbroth dilution method.The biofilm formation level of the strains was determined by semi-quantitative crystal purple method.The biofilm model of Acinetobacter baumannii was constructed by 96-well polystyrene plate in vitro.After the mature biofilm was formed,imipenem at subinhibitory concentration was applied to the mature biofilm.The mature biofilms in experimental groupsⅠ,ⅡandⅢwere treated with imipenem of MIC,1/2MIC and 1/4MIC,respectively.Control groupⅣdid not receive imipenem.The biofilm was stained by crystalline violet semi-quantitative staining,and the biofilm staining was observed under a confocal inverted microscope.The OD value of the biofilm was measured at 570 nm by a multifunctional enzyme plate analyzer.Results:In experimental groupsⅠ,ⅡandⅢ,i.e.MIC,1/2MIC and 1/4MIC,imipenem was applied to the mature biofilm for 48h and 96h,the measured OD values were compared with the control group,and there were significant differences(P<0.05).Conclusion:The subinhibitory concentration of imipenem has the ability to destroy mature biofilm,and the damage ability becomes stronger with the increase of the concentration.

关 键 词：多重耐药鲍曼不动杆菌 生物膜 亚抑菌浓度 亚胺培南 

分 类 号：R965[医药卫生—药理学]