基于16S rDNA测序的皮脂溢出患者肠道菌群分析  

Analysis of intestinal flora in seborrheic patients using 16S rDNA sequencing

作　　者：叶巧园 丁元林 朱坚 江浩波[2] 王琳 YE Qiao-yuan;DING Yuan-ling;ZHU Jian;JIANG Hao-bo;WANG Lin(Guangdong Medical University,Dongguan 523808,China;Donghua Hospital,Dongguan 523110,China)

机构地区：[1]广东医科大学,广东东莞523808 [2]东莞东华医院,广东东莞523110

出　　处：《广东医科大学学报》2021年第4期389-394,共6页Journal of Guangdong Medical University

基　　金：东莞市社会科技发展(一般)项目(No.2018507150461226)。

摘　　要：目的探讨皮脂溢出患者肠道菌群变化。方法收集10例皮脂溢出患者(研究组)及10例健康对照者(对照组)粪便样本,提取菌群DNA,对V3-V4可变区进行扩增及文库构建,采用Illumina Miseq测序平台进行测序,对样本物种丰度和样本间门、科、属、种等水平上的差异进行统计分析,并用PICUSt软件预测差异功能通路。结果研究组肠道微生物群落丰度、多样性相对降低。研究组梭杆菌门(Fusobacteria)、拟杆菌科(Bacteroidaceae)、拟杆菌属(Bacteroides)、布氏瘤胃球菌(Ruminococcus bromii)丰度显著降低,而肠杆菌科(Enterobacteriaceae)、粪便杆菌属(Faecalibacterium)、普拉氏梭杆菌(Faecalibacterium prausnitzii)、活泼瘤胃球菌(Ruminococcus gnavus)、大肠杆菌(Escherichia coli)升高。5条功能通路(ATP结合盒转运蛋白、嘌呤代谢、精氨酸和脯氨酸代谢、精氨酸生物合成、癌症中枢碳代谢)在两组间存在显著差异(P<0.05)。结论胃肠功能紊乱、抑郁与中年皮脂溢出可能有着密切关系。Objective To study the change of intestinal flora in seborrheic patients.Methods The fecal samples were collected from 10 seborrheic patients and 10 healthy controls.The V3-V4 variable region was amplified from extracted bacterial DNA,followed by library construction and Illumina Miseq sequencing.The Phyla,family,genus and species,as well as species abundance were compared between two groups.The functional pathways were predicted by PICUSt software.Results The species abundance and diversity of intestinal microflora were decreased in seborrheic patients.Compared with control group,the abundance of Fusobacteria,Bacteroidaceae,Bacteroides,and Ruminococcus bromii were lower,while Enterobacteriaceae,Faecalibacterium,Faecalibacterium prausnitzii,Ruminococcus gnavus,and Escherichia coli were higher in seborrheic patients.Five functional pathways(ABC transporters,purine metabolism,arginine and proline metabolism,arginine biosynthesis,and central carbon metabolism in cancer)were different between two groups(P<0.05).Conclusion Gastrointestinal dysfunction and depression may be associated with seborrhea in middle-aged individuals.

关 键 词：皮脂溢出 肠道菌群 16S rDNA 

分 类 号：R758.73[医药卫生—皮肤病学与性病学]