嘉兴市异性商业性行为人群HIV分子传播网络特征分析  

Characteristics of HIV transmission network among people with commercial heterosexual contact in Jiaxing

作　　者：葛锐[1] 朱国英[1] 罗明宇 胡洁[1] 宋建强[3] 张佳峰[2] 燕勇[1] 陈中文[1] 潘晓红[2] Ge Rui;Zhu Guoying;Luo Mingyu;Hu Jie;Song Jianqiang;Zhang Jiafeng;Yan Yong;Chen Zhongwen;Pan Xiaohong(Department of AIDS/STD/Tuberculosis Prevention and Control,Jiaxing Prefectural Center for Disease Control and Prevention,Jiaxing 314050,China;Department of AIDS/STD Prevention and Control,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China;Department of AIDS/STD/Tuberculosis Prevention and Control,Haiyan County Center for Disease Control and Prevention,Jiaxing 314300,China)

机构地区：[1]嘉兴市疾病预防控制中心艾滋病性病结核病预防控制科,浙江嘉兴314050 [2]浙江省疾病预防控制中心艾滋病性病防治所,杭州310051 [3]海盐县疾病预防控制中心艾滋病性病结核病预防控制科,浙江嘉兴314300

出　　处：《国际流行病学传染病学杂志》2021年第3期187-190,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省医药卫生科技计划(2019RC292);嘉兴市公益性研究计划(2020AY30022)。

摘　　要：目的应用分子流行病学技术构建嘉兴市经异性商业性行为感染HIV病例的传播网络,并了解其传播特征。方法对2015年1月至2019年6月嘉兴市某县新报告的异性商业性行为HIV病例及其阳性配偶进行流行病学调查。采集血浆并提取RNA,运用RT-PCR和巢式PCR扩增HIV-1的pol区基因,使用MEGA 6.0软件构建系统进化树进行亚型分析,计算毒株间遗传距离。使用Cytoscape软件绘制分子传播网络。结果本次研究共纳入41例HIV病例,以50岁以上老年男性为主(73.2%)。41例病例的HIV-1亚型包括CRF01_AE(34.1%)、CRF07_BC(46.3%)和CRF08_BC(19.5%),其中33例形成7个分子簇,成簇比例为80.5%。在较大分子簇A和C中各发现1例高传播风险病例。结论异性商业性行为人群HIV-1亚型多样,传播特征复杂,成簇病例可能是导致该县艾滋病疫情上升的重要因素。Objective To construct the transmission network of HIV cases infected through commercial heterosexual behavior in Jiaxing by molecular epidemiology technique,and understand its transmission characteristics.Methods The newly reported HIV cases with commercial heterosexual behavior and their positive spouses were investigated in Jiaxing during January 2015 to June 2019.Plasma samples were collected to extract RNA,and the pol gene of HIV-1 was amplified by RT-PCR and nested PCR.The phylogenetic tree was constructed by MEGA 6.0 for subtype analysis.The genetic distance between strains was calculated and the molecular transmission network was drawn by Cytoscape.Results A total of 41 HIV cases were included in this study.Most of them were males over 50 years old(73.2%).HIV-1 subtypes in 41 cases were CRF01_AE(34.1%),CRF07_BC(46.3%)and CRF08_BC(19.5%),and 33 cases formed 7 molecular clusters(80.5%).One case at high risk for HIV transmission was found in the large cluster A and C,respectively.Conclusions The diversity of HIV-1 subtypes among commercial heterosexual behavior people leads to the complexity of transmission.Cluster cases may be a key point leading to the rise of AIDS epidemic in Jiaxing.

关 键 词：HIV 分子流行病学 传播网络 精准干预 遗传 毒株亚型 

分 类 号：R512.91[医药卫生—内科学] R181.3[医药卫生—临床医学]